Seronero L C, Fernández Laespada M E, Pavón J L, Moreno Cordero B
Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Química, Universidad de Salamanca, Spain.
J Chromatogr A. 2000 Nov 3;897(1-2):171-6. doi: 10.1016/s0021-9673(00)00815-3.
The potential of the cloud point methodology for the preconcentration of relatively polar compounds was studied using the non-ionic surfactant Triton X-114 and five EPA chlorophenols as test analytes. Analyte determination was performed using reversed-phase gradient LC with electrochemical and spectrophotometric detection. The amount of surfactant used is a critical variable in the preconcentration factor because it determines the extraction yield and the volume of surfactant-rich phase obtained. These values were determined as a function of the Triton X-114 concentration, together with the phase ratio, which allows prediction of the maximum preconcentration factor under given conditions.
使用非离子表面活性剂吐温X-114和五种美国环保署规定的氯酚作为测试分析物，研究了浊点法对相对极性化合物进行预富集的潜力。采用反相梯度液相色谱法结合电化学和分光光度检测法对分析物进行测定。所用表面活性剂的量是预富集因子中的一个关键变量，因为它决定了萃取产率和所得富表面活性剂相的体积。这些值是作为吐温X-114浓度的函数测定的，同时还测定了相比，这使得能够预测给定条件下的最大预富集因子。
