Pasternak J R, Woolsey T A
J Comp Neurol. 1975 Apr 1;160(3):291-306. doi: 10.1002/cne.901600303.
We located the corresponding barrel in Layer IV of the mouse SmI cortex in eleven cerebral hemispheres sectioned in a plane tangential to the pia overlying SmI and in one sectioned and prepared by a combined Golgi-Nissl method. In the section in which barrel C-1 could be optimally visualized each neuronal soma was outlined with a camera lucida and the cross-sectional area measured with the aid of a small computer. In all, nearly 7,000 neurons were measured. We estimate that on average barrel C-1 contains about 2,000 neurons. The mean cross-sectional area of the perikarya is 62.51 mu2 (S.D. plus or minus 14.51 mu2) and the size distribution of the neurons is unimodal and positively skewed. There is no segregation of cells within the barrel on the basis of size. The spatial distribution of cells in the barrel is fairly constant, from specimen to specimen, and the charactieristic cytoarchitectonic appearance of the barrel can be related to regional neuronal packing density since there are at least 1.6 as many neurons in the sides of the barrel as the hollow. The constancy of the cellular composition of the barrels indicates that the mechanisms responsible for the development of the mouse SmI cortex are fairly rigidly determined, and that the barrel field should lend itself well to further quantitative, developmental and physiological analysis.
我们在11个大脑半球的小鼠初级体感皮层IV层中找到了对应的桶状结构，这些大脑半球是在与覆盖初级体感皮层的软脑膜相切的平面上切片的，还有一个是通过高尔基-尼氏联合方法切片并制备的。在能最佳观察到C-1桶状结构的切片中，用明视野显微镜勾勒出每个神经元胞体，并借助小型计算机测量其横截面积。总共测量了近7000个神经元。我们估计，平均而言，C-1桶状结构包含约2000个神经元。核周体的平均横截面积为62.51μm²（标准差为±14.51μm²），神经元的大小分布呈单峰且正偏态。桶状结构内的细胞不存在基于大小的分离。从一个标本到另一个标本，桶状结构内细胞的空间分布相当恒定，并且桶状结构的特征性细胞结构外观可能与区域神经元堆积密度有关，因为桶状结构侧面的神经元数量至少是中空部分的1.6倍。桶状结构细胞组成的恒定性表明，负责小鼠初级体感皮层发育的机制相当严格地被确定，并且桶状结构应该很适合进一步进行定量、发育和生理学分析。
