Mathé A A, Levine B I, Antonucci M J
J Allergy Clin Immunol. 1975 Mar;55(3):170-9. doi: 10.1016/0091-6749(75)90013-5.
Effects of cortisol and anaphylaxis on the uptake of catecholamines (CA) in the guinea pig lung have not been investigated previously. Sensitized and healthy animals were randomly killed, catheters were inserted into the pulmonary artery and vein, and the preparation was perfused with Tyrode. Half of the animals received 50 mg. of cortisol 2 hours before the procedure. H3-epinephrine (E) or norepinephrine (NE), 10 ng. per milliliter, was infused for 6 minutes. Infusion was started 15 seconds prior to challenge with ovalbumin or NaCl. Total -H3 and NE(E)-H3 were determined in the lung homogenates. Results showed (1) 6 to 14 per cent of circulating CA were retained by the lung. (2) In healthy animals cortisol inhibited NE uptake by 35 per cent and E uptake by 15 per cent. (3) Anaphylaxis increased NE and E accumulation by 10 and 19 per cent, respectively. (4) Regardless of experimental conditions approximately 40 per cent of NE and 30 per cent of E taken up were recovered unchanged.
(1) There is significant uptake of CA in the lung. (2) One of the mechanisms of therapeutic effect of cortisol in asthma might be its inhibitory effect on CA uptake. (3) Increased CA accumulation during anaphylaxis could be beneficial by increasing the local concentration of amines, or detrimental by decreasing availability of CA, depending on the uptake site and cell type and degree of subsequent metabolism. (4) Cortisol and anaphylaxis per se appear not to change degradation of CA.
皮质醇和过敏反应对豚鼠肺组织摄取儿茶酚胺（CA）的影响此前尚未进行过研究。将致敏动物和健康动物随机处死，将导管插入肺动脉和肺静脉，并用台氏液灌注该标本。一半动物在操作前2小时接受50毫克皮质醇。以每毫升10纳克的剂量注入H3 - 肾上腺素（E）或去甲肾上腺素（NE），持续6分钟。在以卵清蛋白或氯化钠进行激发前15秒开始注入。测定肺匀浆中的总 -H3和NE（E）-H3。结果显示：（1）肺组织保留了6%至14%的循环CA。（2）在健康动物中，皮质醇使NE摄取减少35%，使E摄取减少15%。（3）过敏反应使NE和E的蓄积分别增加了10%和19%。（4）无论实验条件如何，摄取的NE约40%和E约30%回收时未发生变化。
（1）肺组织对CA有显著摄取。（2）皮质醇在哮喘治疗中的作用机制之一可能是其对CA摄取的抑制作用。（3）过敏反应期间CA蓄积增加可能是有益的，可增加胺类的局部浓度，也可能是有害的，会降低CA的可用性，这取决于摄取部位、细胞类型以及后续代谢程度。（4）皮质醇和过敏反应本身似乎不会改变CA的降解。
