利用乙酰栖热放线菌的鸟苷-次黄苷激酶对鸟苷进行磷酸化并结合ATP再生。

Phosphorylation of guanosine using guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.

作者信息

Kawasaki H, Usuda Y, Shimaoka M, Utagawa T

机构信息

Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc., Kawasaki-shi, Japan.

出版信息

Biosci Biotechnol Biochem. 2000 Oct;64(10):2259-61. doi: 10.1271/bbb.64.2259.

DOI:10.1271/bbb.64.2259

PMID:11129609

Abstract

Guanosine 5'-monophosphate (5'-GMP) and inosine 5'-monophosphate (5'-IMP) are widely used as flavor enhancers. Recently, a novel process for 5'-IMP production by phosphorylation of inosine using guanosine-inosine kinase coupled with ATP regeneration was reported. In this study, we demonstrated the practical possibility of producing 5'-GMP by phosphorylation of guanosine using a guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.

摘要

5'-鸟苷酸（5'-GMP）和5'-肌苷酸（5'-IMP）被广泛用作增味剂。最近，有报道称一种利用鸟苷-肌苷激酶与ATP再生相结合通过肌苷磷酸化生产5'-IMP的新方法。在本研究中，我们证明了利用来自乙酰栖热菌的鸟苷-肌苷激酶与ATP再生相结合通过鸟苷磷酸化生产5'-GMP的实际可能性。