Paily K P, Balaraman K
Vector Control Research Centre (Indian Council of Medical Research), Pondicherry, India.
Med Vet Entomol. 2000 Dec;14(4):426-9. doi: 10.1046/j.1365-2915.2000.00263.x.
Ten species of mosquitoes (Diptera: Culicidae) from five genera were exposed to preparasites of the tropical mermithid nematode species Romanomermis iyengari (Welch) (Nematoda: Mermithidae), a strain isolated in 1978 from Pondicherry. By exposing mosquito larvae during the second instar, nematode infection was invariably lethal, the rate being highest in Culex sitiens Wiedemann (95%) followed by Cx. quinquefasciatus Say (90%), Aedes aegypti (L.) (79%), Anopheles subpictus Grassi (64%), Ae. albopictus (Skuse) and Armigeres subalbatus Coquillett (62%), Cx. tritaeniorhynchus Giles (57%), Mansonia annulifera (Theobald) (46%), An. stephensi Liston (40%) and An. culicifacies Giles (36%). When fourth-instar larvae were exposed, the infection was highest in Ar. subalbatus (66%), followed by An. stephensi (52%), Cx. quinquefasciatus (47%), Ae. aegypti and An. subpictus (42%), Ae. albopictus (30%), An. culicifacies (29%), Cx. sitiens (24%), Cx. tritaeniorhynchus (19%) and Ma. annulifera (8%), with 2-45% of infected culicines surviving to adulthood. The parasitic phase of the nematode lasted 5-7 days in all the host species, yielding 1.1-3.2 parasites per II instar and 1.1-2.5 parasites per IV instar. The overall output of parasites per 100 mosquito larvae (infected + uninfected) was highest for Ae. aegypti when mosquitoes were exposed during II instar (2.53 parasites/larva) and for Ar. subalbatus when mosquitoes were exposed during IV instar (1.65/larva), and lowest for Ma. annulifera exposed during IV instar (0.09/larva). For routine laboratory culture of R. iyengari it is convenient to employ Cx. quinquefasciatus as the host yielding 90-190 parasites/100 larva.
来自五个属的十种蚊子（双翅目：蚊科）被暴露于热带索线虫属线虫罗曼诺索线虫（Romanomermis iyengari）（韦尔奇）（线虫纲：索线虫科）的前寄生虫中，该菌株于1978年从本地治里分离得到。通过在第二龄期暴露蚊子幼虫，线虫感染总是致命的，感染率最高的是尖音库蚊（Culex sitiens）维德曼（95%），其次是致倦库蚊（Cx. quinquefasciatus）赛伊（90%）、埃及伊蚊（Aedes aegypti）（L.）（79%）、伪杂鳞库蚊（Anopheles subpictus）格拉西（64%）、白纹伊蚊（Ae. albopictus）（斯库斯）和骚扰阿蚊（Armigeres subalbatus）科奎利特（62%）、三带喙库蚊（Cx. tritaeniorhynchus）吉尔斯（57%）、环带曼蚊（Mansonia annulifera）（西奥博尔德）（46%）、斯氏按蚊（An. stephensi）利斯顿（40%）和库氏按蚊（An. culicifacies）吉尔斯（36%）。当暴露四龄幼虫时，感染率最高的是骚扰阿蚊（66%），其次是斯氏按蚊（52%）、致倦库蚊（47%）、埃及伊蚊和伪杂鳞库蚊（42%）、白纹伊蚊（30%）、库氏按蚊（29%）、尖音库蚊（24%）、三带喙库蚊（19%）和环带曼蚊（8%），2 - 45%的受感染库蚊能存活至成年。线虫的寄生阶段在所有宿主物种中持续5 - 7天，每二龄幼虫产生1.1 - 3.2条寄生虫，每四龄幼虫产生1.1 - 2.5条寄生虫。当在二龄期暴露蚊子时，每100只蚊子幼虫（感染 + 未感染）的寄生虫总产出以埃及伊蚊最高（2.53条寄生虫/幼虫），当在四龄期暴露蚊子时，以骚扰阿蚊最高（1.65条/幼虫），而在四龄期暴露的环带曼蚊最低（0.09条/幼虫）。对于罗曼诺索线虫的常规实验室培养，使用致倦库蚊作为宿主很方便，每100只幼虫可产生90 - 190条寄生虫。
