Adams E, Rafiee M, Roets E, Hoogmartens J
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
J Pharm Biomed Anal. 2000 Dec 15;24(2):219-26. doi: 10.1016/s0731-7085(00)00413-1.
The analysis of streptomycin sulfate using a column packed with base deactivated reversed phase silica gel and ultraviolet (UV) detection at 205 nm is described. The mobile phase consists of an aqueous solution containing 14 g/l of sodium sulfate, 1.5 g/l of sodium octanesulfonate, 50 ml/l of acetonitrile and 50 ml/l of a 0.2 M phosphate buffer at pH 3.0. The method allows separating streptidine, streptomycin B, streptomycin and dihydrostreptomycin, as well as several other components, which were not yet identified. The total time of analysis is 50 min. The effects of the different chromatographic parameters on the separation were investigated. A number of commercial samples were analyzed using this method.
描述了使用填充有碱钝化反相硅胶的色谱柱并在205nm处进行紫外（UV）检测来分析硫酸链霉素的方法。流动相由含有14g/l硫酸钠、1.5g/l辛烷磺酸钠、50ml/l乙腈和50ml/l pH 3.0的0.2M磷酸盐缓冲液的水溶液组成。该方法能够分离链霉胍、链霉素B、链霉素和二氢链霉素，以及其他几种尚未鉴定的成分。分析总时间为50分钟。研究了不同色谱参数对分离的影响。使用该方法分析了多个商业样品。
