Demski L S, Bauer D H, Gerald J W
J Exp Zool. 1975 Feb;191(2):215-32. doi: 10.1002/jez.1401910209.
Acute brain stimulation experiments were carried out in anesthetized male green sunfish, Lepomis cyanellus. Semen discharge was evoked consistently by low level electrical stimulation (100 muA or less) in the following areas: the preoptic region, dorsal hypothalamus, thalamus, midbrain tegmentum and the basolateral midbrain and medulla. Areas which were stimulated repeatedly at 100 muA and were always negative for sperm release included: the telencephalon with the exception of the preoptic region, the optic tectum, the cerebellum, the inferior lobe of the hypothalamus, the nucleus rotundus and the dorsal medulla. Removal of most of the optic tectum and cerebellum failed to block reponses evoked from the preoptic area; however, they were usually eliminated by transecting the rostral spinal cord. Electrical stimulation of an isolated 4 mm segment of spinal cord located at the third vertebral level resulted in sperm release, indicating that adequate mechanisms for semen discharge are present within the upper spinal cord. The results of this study suggest that a sperm release system in the green sunfish extends from the preoptic area to the spinal cord passing through the hypothalamus, midbrain tegmentum and basal midbrain and medulla.
在麻醉的雄性绿太阳鱼（蓝鳃太阳鱼）身上进行了急性脑刺激实验。在以下区域，低水平电刺激（100微安或更低）能持续诱发精液排出：视前区、背侧下丘脑、丘脑、中脑被盖以及中脑基底外侧和延髓。以100微安反复刺激但对精子释放始终呈阴性的区域包括：除视前区外的端脑、视顶盖、小脑、下丘脑下叶、圆核和背侧延髓。切除大部分视顶盖和小脑未能阻断从视前区诱发的反应；然而，这些反应通常通过横断脊髓头端而消除。对位于第三椎骨水平的一段4毫米孤立脊髓进行电刺激会导致精子释放，这表明脊髓上部存在足够的精液排出机制。这项研究的结果表明，绿太阳鱼的精子释放系统从视前区延伸至脊髓，途经下丘脑、中脑被盖、中脑基底和延髓。
