Pallini R, Casalbore P, Mercanti D, Maggiano N, Larocca L M
Institute of Neurosurgery, CNR, Rome, Italy.
J Neurooncol. 2000 Aug;49(1):9-17. doi: 10.1023/a:1006436903976.
One objection to using cell cultures for studying the proliferation of tumors is the potential for phenotypic changes that may occur in vitro. Here, we compared the antigen pattern expression of cultured meningioma cells with that of the primary tumor. Cell cultures established from 9 intracranial meningiomas and deparaffinized sections of the resected tumors were analyzed for immunophenotyping with the following antibodies: vimentin, cytokeratin, epithelial membrane antigen, S-100, neuron-specific enolase, synaptophisin, factor VIII-related antigen, CD4, CD31, CD34, CD45RB, CD68-PGM1, CD68-KP, and myeloid/histiocyte antigen (MAC387). Overall, the cultured meningioma cells retained the main feature of the primary tumor, being positive both for mesenchymal antigens and for epithelial antigens. Interestingly, the cultured meningioma cells abundantly expressed the CD68 antigens at early passage. The CD68 antigens, which are normally found on hematopoietic cells like macrophages and monocytes, were not detectable on meningioma cells in situ. Our results show that phenotypic changes on human meningioma cells may occur in vitro. This phenomenon suggests caution when transposing the in vitro results to the in vivo condition.
对于使用细胞培养来研究肿瘤增殖的一个反对观点是体外可能发生表型变化。在此，我们比较了培养的脑膜瘤细胞与原发性肿瘤的抗原模式表达。对从9例颅内脑膜瘤建立的细胞培养物以及切除肿瘤的脱石蜡切片用以下抗体进行免疫表型分析：波形蛋白、细胞角蛋白、上皮膜抗原、S-100、神经元特异性烯醇化酶、突触素、因子VIII相关抗原、CD4、CD31、CD34、CD45RB、CD68-PGM1、CD68-KP和髓样/组织细胞抗原（MAC387）。总体而言，培养的脑膜瘤细胞保留了原发性肿瘤的主要特征，对间充质抗原和上皮抗原均呈阳性。有趣的是，培养的脑膜瘤细胞在传代早期大量表达CD68抗原。CD68抗原通常在巨噬细胞和单核细胞等造血细胞上发现，而原位脑膜瘤细胞上无法检测到。我们的结果表明人脑膜瘤细胞在体外可能发生表型变化。这种现象表明在将体外结果转换为体内情况时应谨慎。
