Kimura T, Suzuki S, Yoshida A
J Nutr. 1975 Feb;105(2):257-65. doi: 10.1093/jn/105.2.257.
To study the effect of an essential amino acid deficiency on gastrointestinal functions, rats were force-fed a 10% amino acid diet devoid of valine for several days, and subsequently a complete amino acid diet. Force-feeding of the valine-free diet for a few days delayed the disappearance of dietary nitrogen from the gastrointestinal tract. ASINGLE FEEDING OF THE COMPLETE AMINO ACID DIET CONTAINING 14C-labeled amino acids or (14C)glucose to rats previously fed the valine-free diet did not improve disappearance of the nitrogen. The absorption of the dietary carbohydrate as well as dietary nitrogen was delayed after feeding the valine-free diet. In spite of the delayed disappearance of dietary 14C-labeled amino acids or (14C)glucose from the gastrointestinal tract, the incorporation of these dietary 14C-labeled amino acids into liver lipids was not different between rats fed the complete amino acid diet and those fed the valine-free diet. Under these experimental conditions, changes in disaccharidase activities in the intestinal mucosa were in parallel with changes in the disappearance of nutrients from the gastrointestinal tract.
为研究必需氨基酸缺乏对胃肠功能的影响，给大鼠强制喂食不含缬氨酸的10%氨基酸饮食数天，随后喂食完全氨基酸饮食。强制喂食不含缬氨酸的饮食数天会延迟膳食氮从胃肠道的消失。给先前喂食不含缬氨酸饮食的大鼠单次喂食含14C标记氨基酸或（14C）葡萄糖的完全氨基酸饮食，并不会改善氮的消失情况。喂食不含缬氨酸的饮食后，膳食碳水化合物以及膳食氮的吸收均延迟。尽管膳食中14C标记氨基酸或（14C）葡萄糖从胃肠道的消失延迟，但喂食完全氨基酸饮食的大鼠与喂食不含缬氨酸饮食的大鼠相比，这些膳食中14C标记氨基酸掺入肝脏脂质的情况并无差异。在这些实验条件下，肠黏膜中二糖酶活性的变化与营养素从胃肠道消失的变化平行。
