p2Sp1 RNA在镁离子和非离子去污剂（Brij 58）作用下的自我切割

Self-cleavage of p2Sp1 RNA with Mg2+ and non-ionic detergent (Brij 58).

作者信息

Hosaka H, Hosono K, Kawai G, Takai K, Takaku H

机构信息

Department of Industrial Chemistry and High Technology Research Center, Chiba Institute of Technology, Narashino, Japan.

出版信息

J Inorg Biochem. 2000 Nov;82(1-4):215-9. doi: 10.1016/s0162-0134(00)00164-1.

DOI:10.1016/s0162-0134(00)00164-1

PMID:11132630

Abstract

The precursor of an RNA molecule from T4-infected E. coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions [(UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. We studied the self-cleavage reaction of an RNA fragment (GUUUCGUACAAAC) (R1) with the sequence corresponding to the p2Sp1 RNA in the presence of Mg2+ and non-ionic detergents. It requires Mg2+ and is aided by a non-ionic detergent, Brij 58. The cleavage reaction is time, temperature, and pH-dependent. The cleavage occurs at the phosphodiester bond between UpA and CpA on the RNA fragment (GUUUCGUACAAAC) (R1). Furthermore, the maximum of cleavage of R1 occurs at a very low Mg2+ concentration (< or = 5 mM).

摘要

来自T4噬菌体感染的大肠杆菌细胞的RNA分子前体（p2Sp1 RNA）能够在假定的环和茎结构内的特定位置[（UpA（139 - 140）和CpA（170 - 171）]进行自我切割。这种序列特异性切割至少需要一价阳离子和非离子去污剂。我们研究了在Mg2+和非离子去污剂存在下，与p2Sp1 RNA序列对应的RNA片段（GUUUCGUACAAAC）（R1）的自我切割反应。它需要Mg2+，并受到非离子去污剂Brij 58的辅助。切割反应与时间、温度和pH有关。切割发生在RNA片段（GUUUCGUACAAAC）（R1）上UpA和CpA之间的磷酸二酯键处。此外，R1的最大切割发生在非常低的Mg2+浓度（≤5 mM）下。