能够形成茎环结构的寡核糖核苷酸的序列特异性切割。

Sequence-specific cleavage of oligoribonucleotide capable of forming a stem and loop structure.

作者信息

Hosaka H, Sakabe I, Sakamoto K, Yokoyama S, Takaku H

机构信息

Department of Industrial Chemistry, Chiba Institute of Technology, Japan.

出版信息

J Biol Chem. 1994 Aug 5;269(31):20090-4.

PMID:8051096

Abstract

The precursor of an RNA molecule from T4-infected Escherichia coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions ((UpA (137-138) and CpA (170-171)) within a possible loop and stem structure. This specific cleavage requires at least a monovalent cation and nonionic detergents. To confirm that the sequence-specific cleavage occurs autolytically, we studied the selective hydrolysis of RNA using a chemically synthesized 13-mer (GUUUCGUACAAAC) having sequences corresponding to G131-C143 of p2Sp1 RNA. The cleavage occurred at two identical sites (UpA and CpA) of a hairpin loop under physiological conditions and was affected by monovalent or divalent metals, nonionic detergent, salt, pH, and temperature. The hairpin loop domain and specific sequences are necessary for the cleavage of RNA 13-mer (GUUUCGUACAAAC).

摘要

来自T4噬菌体感染的大肠杆菌细胞的RNA分子前体（p2Sp1 RNA）能够在可能的环和茎结构内的特定位置（（UpA（137 - 138）和CpA（170 - 171））进行自我切割。这种特异性切割至少需要一价阳离子和非离子去污剂。为了证实序列特异性切割是自动催化发生的，我们使用化学合成的13聚体（GUUUCGUACAAAC）研究了RNA的选择性水解，该13聚体具有与p2Sp1 RNA的G131 - C143对应的序列。在生理条件下，切割发生在发夹环的两个相同位点（UpA和CpA），并且受到一价或二价金属、非离子去污剂、盐、pH和温度的影响。发夹环结构域和特定序列对于RNA 13聚体（GUUUCGUACAAAC）的切割是必需的。