Tanii H, Zang X, Saito N, Saijoh K
Department of Hygiene, Kanazawa University School of Medicine, Kanazawa, Japan.
Brain Res. 2000 Dec 29;887(2):454-9. doi: 10.1016/s0006-8993(00)03035-3.
Nitriles are a class of compounds with potential relevance to human health. Allylnitrile, one of nitriles, induces persistent behavioral abnormalities in mice. To explore what type of neuronal system is involved in these behavioral abnormalities, five neuronal markers, gamma-aminobutyric acid (GABA), tyrosine hydroxylase, serotonin, the serotonin transporter and choline acetyltransferase were immunohistochemically examined within various brain structures in allylnitrile and vehicle-treated mice. Allylnitrile induced changes in the immunolabelling of GABA in the medial habenula, interpeduncular nucleus, substantia nigra, dorsal raphe nucleus and median raphe nucleus; the amount of immunolabelling decreased in all of these brain structures except the medial habenula at 2 days postdosing, and increased in all of these structures at 14 days postdosing. Allylnitrile also induced changes in the amount of immunolabelling of tyrosine hydroxylase in the arcuate nucleus, substantia nigra pars compacta, locus coeruleus and caudoventrolateral reticular nucleus at either 2 or 14 days postdosing, depending on the structures. No immunohistochemical change was seen for serotonin, serotonin transporter and choline acetyltransferase. The present results suggest that the GABAergic systems through the medial habenula-interpeduncular nucleus-ascending raphe nuclei relay and through the substantia nigra may be involved in allylnitrile-induced behavioral abnormalities.
腈类是一类与人类健康可能相关的化合物。烯丙腈作为腈类化合物之一，可诱导小鼠出现持续的行为异常。为探究何种类型的神经系统参与了这些行为异常，我们采用免疫组织化学方法，检测了烯丙腈处理组和溶剂处理组小鼠不同脑区中5种神经标志物，即γ-氨基丁酸（GABA）、酪氨酸羟化酶、5-羟色胺、5-羟色胺转运体和胆碱乙酰转移酶的表达情况。烯丙腈可诱导内侧缰核、脚间核、黑质、中缝背核和中缝正中核中GABA免疫标记的变化；给药后2天，除内侧缰核外，所有这些脑区的免疫标记量均减少，给药后14天，所有这些脑区的免疫标记量均增加。烯丙腈还可诱导弓状核、黑质致密部、蓝斑和尾腹外侧网状核中酪氨酸羟化酶免疫标记量在给药后2天或14天发生变化，具体取决于脑区。5-羟色胺、5-羟色胺转运体和胆碱乙酰转移酶未出现免疫组织化学变化。目前的结果表明，通过内侧缰核-脚间核-中缝核上行传导通路以及黑质的GABA能系统可能参与了烯丙腈诱导的行为异常。
