Li Y, Lou Y, Liang Z, Ren H
Viral Hepatitis Institute of Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhonghua Gan Zang Bing Za Zhi. 2000 Dec;8(6):330-1.
To study the presenting effect of dendritic cell (DC) proliferated from peripheral blood monocytes in patients with chronic hepatitis B on HBsAg.
DC proliferated from patient's peripheral blood monocytes by adding GM-CSF and IL-4 were incubated with HBsAg for 1.5h at different concentrations, then mixed with autologous T cell and cocultured in 96-well flat-bottomed microtiter plates for 5 d at 37 degrees C and 5% CO(2). (3)H-TdR was added 13h before the culture completion, and finally the cells were gathered and the cpm was tested.
The proliferation of T cell by DC treated by HBsAg is significantly higher than untreated.
DC cultured from peripheral blood monocytes in patients with chronic hepatitis B plays a role in presenting HBsAg.
研究慢性乙型肝炎患者外周血单核细胞增殖的树突状细胞（DC）对HBsAg的提呈作用。
通过添加GM-CSF和IL-4从患者外周血单核细胞增殖的DC与不同浓度的HBsAg孵育1.5小时，然后与自体T细胞混合，在96孔平底微量滴定板中于37℃和5% CO₂条件下共培养5天。在培养结束前13小时加入³H-TdR，最后收集细胞并检测cpm。
经HBsAg处理的DC刺激的T细胞增殖明显高于未处理的。
慢性乙型肝炎患者外周血单核细胞培养的DC在提呈HBsAg中起作用。
