采用高效液相色谱法测定不同株系绿僵菌及其经甲磺酸乙酯和紫外线诱导产生的突变体所产生的环肽毒素—— destruxins。

Determination of destruxins, cyclic peptide toxins, produced by different strains of Metarhizium anisopliae and their mutants induced by ethyl methane sulfonate and ultraviolet using HPLC method.

作者信息

Hsiao Y M, Ko J L

机构信息

Pesticide Chemistry Department, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, 11 Kuang-Ming Road, Wufeng, Taichung Hsien 41301, Taiwan, ROC.

出版信息

Toxicon. 2001 Jun;39(6):837-41. doi: 10.1016/s0041-0101(00)00217-8.

DOI:10.1016/s0041-0101(00)00217-8

PMID:11137544

Abstract

Metarhizium anisopliae produces a family of cyclic peptide toxins, destruxins (DTXs), which exhibit various insecticidal activity. Four major DTXs have been separated by HPLC and identified by the liquid chromatography electrospray mass spectrometry (LC-ESI-MS) methods. Strain F061 of M. anisopliae produced large amounts of (DTXs), especially DTX-A (12.84+/-0.04 microg/ml), DTX-B (66.89+/-2.57 microg/ml) and DMDB (1.41+/-0.13 microg/ml). High levels of DTX-E (4.19+/-0.13 microg/ml) were produced by strain F007 of M. anisopliae. The results of our studies also showed that either ethyl methane sulfonate (EMS) or ultraviolet (UV) can significantly increase the production of DTXs. Mutant 61E-9 produced high levels of DTX-A (30.05+/-1.97 microg/ml), DTX-B (110.37+/-10.02 microg/ml) and DMDB (8.30+/-0.45 microg/ml). High levels of DTX-E (20.59+/-2.65 microg/ml) were produced by mutant 7E-3. Both mutant strains are suitable for industrial fermentation processes and possess a wide range of potential applications in the area of metabolic toxin production.

摘要

绿僵菌产生一类环肽毒素—— destruxins（DTXs），它们具有多种杀虫活性。四种主要的 DTXs 已通过高效液相色谱法（HPLC）分离，并通过液相色谱电喷雾质谱法（LC - ESI - MS）进行了鉴定。绿僵菌菌株 F061 产生大量的（DTXs），尤其是 DTX - A（12.84±0.04 μg/ml）、DTX - B（66.89±2.57 μg/ml）和 DMDB（1.41±0.13 μg/ml）。绿僵菌菌株 F007 产生高水平的 DTX - E（4.19±0.13 μg/ml）。我们的研究结果还表明，甲基磺酸乙酯（EMS）或紫外线（UV）均可显著提高 DTXs 的产量。突变体 61E - 9 产生高水平的 DTX - A（30.05±1.97 μg/ml）、DTX - B（110.37±10.02 μg/ml）和 DMDB（8.30±0.45 μg/ml）。突变体 7E - 3 产生高水平的 DTX - E（20.59±2.65 μg/ml）。这两种突变菌株均适用于工业发酵过程，并且在代谢毒素生产领域具有广泛的潜在应用。