环肽毒素的生物合成

Biosynthesis of destruxins.

作者信息

Jegorov A, Sedmera P, Matha V

机构信息

Galena Co., Research Unit, Branisovská, Czech Republic.

出版信息

Phytochemistry. 1993 Aug;33(6):1403-5. doi: 10.1016/0031-9422(93)85099-d.

DOI:10.1016/0031-9422(93)85099-d

PMID:7763948

Abstract

L-[Me-13C]-Methionine, sodium [2-13C]acetate and sodium [1,2-13C]acetate were incorporated into destruxins A, B and E by the entomopathogenic fungus Metarhizium anisopliae. NMR analysis established that methionine is involved in the N-methylation of L-valine and L-alanine, and that one intact acetate unit participates in the biosynthesis of the -CH(OH)-COOH fragment of the hydroxy acid moiety. The presence of two acetate units in proline and isoleucine agrees with the accepted biochemical schemes.

摘要

昆虫病原真菌绿僵菌将L-[甲基-13C]-蛋氨酸、[2-13C]乙酸钠和[1,2-13C]乙酸钠掺入到 destruxins A、B 和 E 中。核磁共振分析表明，蛋氨酸参与了L-缬氨酸和L-丙氨酸的N-甲基化过程，且一个完整的乙酸单元参与了羟基酸部分-CH(OH)-COOH片段的生物合成。脯氨酸和异亮氨酸中存在两个乙酸单元，这与公认的生化途径相符。