Schäfer H, Bernard L, Courties C, Lebaron P, Servais P, Pukall R, Stackebrandt E, Troussellier M, Guindulain T, Vives-Rego J, Muyzer G
FEMS Microbiol Ecol. 2001 Jan;34(3):243-253. doi: 10.1111/j.1574-6941.2001.tb00775.x.
A mesocosm experiment was performed to study the influence of nutrients on activity and diversity of bacterial assemblages from the Mediterranean Sea. Changes in the diversity of the predominant bacterial populations were monitored by DGGE fingerprinting of PCR products derived from 16S rRNA encoding genes. Fluctuations in the diversity of the most active populations was inferred by performing the DGGE fingerprinting on the basis of the cellular rRNA after reverse transcription and PCR amplification. DNA-derived DGGE patterns obtained from duplicate control and nutrient-enriched mesocosms showed differences in the development of the bacterial communities between control and nutrient-enriched experimental mesocosms. Multidimensional scaling analysis of the DNA-derived DGGE fingerprints indicated that duplicate treatments were reproducible. DNA- and RNA-derived DGGE fingerprints of bacterial assemblages changed over time, showing that the composition of the bacterial assemblages, as well as the most active bacterial populations changed during different phases of the incubation. Sequences of predominant DGGE bands in RNA-derived patterns were similar to 16S rRNA gene sequences of members of the alpha-, gamma- and delta-Proteobacteria and of the Cytophaga-Flavobacterium-Bacteroides phylum (CFB). Bands corresponding to Ruegeria-like bacteria and members of the CFB became especially dominant during the course of incubation, suggesting that these populations were important contributors to bacterial production and activity in the post-grazing phase of the experiment.
进行了一项中宇宙实验，以研究营养物质对地中海细菌群落活性和多样性的影响。通过对源自16S rRNA编码基因的PCR产物进行变性梯度凝胶电泳（DGGE）指纹图谱分析，监测主要细菌种群多样性的变化。通过在反转录和PCR扩增后基于细胞rRNA进行DGGE指纹图谱分析，推断最活跃种群多样性的波动情况。从重复的对照和营养富集的中宇宙获得的基于DNA的DGGE图谱显示，对照和营养富集的实验中宇宙之间细菌群落的发展存在差异。对基于DNA的DGGE指纹图谱进行的多维尺度分析表明，重复处理具有可重复性。细菌群落基于DNA和RNA的DGGE指纹图谱随时间变化，表明在培养的不同阶段，细菌群落的组成以及最活跃的细菌种群都发生了变化。基于RNA的图谱中主要DGGE条带的序列与α-、γ-和δ-变形菌门以及噬纤维菌-黄杆菌-拟杆菌门（CFB）成员的16S rRNA基因序列相似。在培养过程中，与类鲁杰氏菌和CFB成员相对应的条带变得尤为占优势，这表明这些种群是实验后放牧阶段细菌生产和活性的重要贡献者。
