Singh D K, Jagdish S, Gautam O P, Dhar S
Trop Anim Health Prod. 1979 May;11(2):87-90. doi: 10.1007/BF02237776.
Groups of Theileria annulata infected Hyalomma anatolicum anatolicum adults prefed on a calf for 1 to 6 days were separately ground in tissue culture medium-199, supplemented with bovine albumin powder, Fraction-V. Supernatant fluid was collected, made up with additional medium, so that each millilitre represented material from 25 ticks, and was injected subcutaneously into groups of cross-bred male calves. The results indicated that ground-up tick supernate (GUTS) prepared from unfed ticks was not infective, whereas that prepared from 1 to 6 days prefed ticks was infective. GUTS prepared from 3 days prefed ticks appeared to contain highest infectivity and 1 ml of it induced fatal theileriosis.
将已在小牛身上预饲1至6天的环形泰勒虫感染的安纳托利亚璃眼蜱成虫分组，分别在添加了牛血清白蛋白粉末（第五组分）的199组织培养基中研磨。收集上清液，用额外的培养基补足，使每毫升代表25只蜱的材料，并皮下注射到杂交雄性小牛组中。结果表明，从未进食的蜱制备的蜱研磨上清液（GUTS）没有感染性，而从预饲1至6天的蜱制备的GUTS具有感染性。从预饲3天的蜱制备的GUTS似乎含有最高的感染性，1毫升就能诱发致命的泰勒虫病。
