Bae J S, Kim D Y, Hwang W S, Kim J H, Lee N S, Nam H W
Department of Pathology, College of Verterinary Medicine, Seoul National University, Suwon City 441-744, Korea.
Korean J Parasitol. 2000 Dec;38(4):245-9. doi: 10.3347/kjp.2000.38.4.245.
A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.
采用IgG-ELISA法对492份牛血清进行了犬新孢子虫（Nc-1株和韩国分离株KBA-2）及刚地弓形虫检测。在492份血清中，113份血清（23.0%）对犬新孢子虫的Nc-1株或KBA-2株呈阳性反应。在这113份阳性血清中，92份血清（81.4%）与两种菌株的抗原均发生反应，但6份血清（5.3%）仅与Nc-1株反应，15份血清（13.3%）仅与KBA-2株反应。对于刚地弓形虫抗原，6份血清（1.2%）呈阳性，但所有这些血清也与犬新孢子虫抗原发生反应。免疫印迹显示，根据ELISA值呈现典型的结合模式，即高OD值组特异性地与包括43 kDa蛋白在内的主要表面蛋白发生反应。23.0%的血清阳性率表明新孢子虫病似乎是牛流产的主要原因之一。建议在全国范围内系统地开展更多的流行病学研究。
