Bartko J
Vet Med (Praha). 1979 May;24(5):285-94.
The author describes the serological demonstration of toxoplasma antibodies in sheep of ten flocks kept in the lowland region of western Slovakia. The results of serological examination by the complement-fixation reaction (CFR) were positive in different degrees in nine flocks. In the remaining flock the blood of the sheep was not found to contain such antibodies. At the locality L. the author selected a small group of serologically positive sheep from the group of animals to be slaughtered and he studied the CFR values of antibodies in the course of nine months. Toxoplasma strain denoted as H-1 was isolated from the brain of a ram of this group which had a 1 : 256 antibody titre in the blood. This strain was found to be highly virulent for white mice. Toxoplasma antibodies were also identified in the blood of exposed workers, in the blood of dogs, mice and sewer rats. The patho-anatomic picture and isolation of toxoplasma strains from the brain of dead sheep or their foetuses which had the antibodies in the blood before death -- all this demonstrated the occurrence of congenital infection.
作者描述了在斯洛伐克西部低地地区饲养的10个羊群的绵羊中弓形虫抗体的血清学检测情况。通过补体结合反应(CFR)进行的血清学检查结果显示,9个羊群中不同程度地呈阳性。在其余的羊群中,未发现绵羊血液中含有此类抗体。在L地,作者从待屠宰的动物群体中挑选了一小群血清学检测呈阳性的绵羊,并在9个月的时间里研究了抗体的CFR值。从该群体中一只血液抗体滴度为1:256的公羊大脑中分离出了标记为H-1的弓形虫菌株。发现该菌株对白鼠具有高致病性。在接触过的工人血液、狗、小鼠和褐家鼠的血液中也鉴定出了弓形虫抗体。病理解剖情况以及从死前血液中含有抗体的死羊或其胎儿大脑中分离出弓形虫菌株——所有这些都证明了先天性感染的发生。