使用Northern印迹法对mRNA水平进行定量分析。 - Suppr

Quantification of mRNA levels using northern blotting.

作者信息

Fernyhough P

机构信息

Department of Pharmacology, Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London, London, UK.

出版信息

Methods Mol Biol. 2001;169:53-63. doi: 10.1385/1-59259-060-8:53.

DOI:10.1385/1-59259-060-8:53

PMID:11142020

Abstract

摘要

相似文献

Quantification of mRNA levels using northern blotting.

Methods Mol Biol. 2001;169:53-63. doi: 10.1385/1-59259-060-8:53.

Detection and assay of nerve growth factor mRNA.

Methods Enzymol. 1991;198:48-61. doi: 10.1016/0076-6879(91)98007-s.

Quantitative and sensitive northern blot hybridization using PCR-generated DNA probes labeled with digoxigenin by nick translation.

Biotechniques. 1993 Nov;15(5):880-2.

Quantification of neurotrophin mRNA by RT-PCR.

Methods Mol Biol. 2001;169:81-90. doi: 10.1385/1-59259-060-8:81.

Quantification of mRNA in neuronal tissue by northern analysis.

Methods Mol Med. 2003;79:161-79. doi: 10.1385/1-59259-358-5:161.

Construction of transgenic animals overproducing neurotrophins and their receptors.

Methods Mol Biol. 2001;169:167-91. doi: 10.1385/1-59259-060-8:167.

Northern blot analysis.

Methods Mol Biol. 1996;58:113-28. doi: 10.1385/0-89603-402-X:113.

Analysis of gene expression by northern blot.

Mol Biotechnol. 1994 Dec;2(3):227-42. doi: 10.1007/BF02745879.

Quantification of mRNA levels using ribonuclease protection assay.

Methods Mol Biol. 2001;169:65-79. doi: 10.1385/1-59259-060-8:65.

Northern blotting of RNA denatured in glyoxal without buffer recirculation.

Biotechniques. 1997 Apr;22(4):668-71. doi: 10.2144/97224st01.

引用本文的文献

PTT-quant: a new method for direct identification and absolute quantification of premature transcription termination events, following the example of bacterial riboswitches.

Appl Microbiol Biotechnol. 2022 Feb;106(4):1557-1570. doi: 10.1007/s00253-022-11809-1. Epub 2022 Feb 5.

Errors in CGAP xProfiler and cDNA DGED: the importance of library parsing and gene selection algorithms.

BMC Bioinformatics. 2011 Apr 15;12:97. doi: 10.1186/1471-2105-12-97.

Sizing up the future of microRNA analysis.

Anal Bioanal Chem. 2010 Nov;398(6):2535-49. doi: 10.1007/s00216-010-4018-8. Epub 2010 Aug 1.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

相似文献

Quantification of mRNA levels using northern blotting.

Methods Mol Biol. 2001;169:53-63. doi: 10.1385/1-59259-060-8:53.

Detection and assay of nerve growth factor mRNA.

Methods Enzymol. 1991;198:48-61. doi: 10.1016/0076-6879(91)98007-s.

Quantitative and sensitive northern blot hybridization using PCR-generated DNA probes labeled with digoxigenin by nick translation.

Biotechniques. 1993 Nov;15(5):880-2.

Quantification of neurotrophin mRNA by RT-PCR.

Methods Mol Biol. 2001;169:81-90. doi: 10.1385/1-59259-060-8:81.

Quantification of mRNA in neuronal tissue by northern analysis.

Methods Mol Med. 2003;79:161-79. doi: 10.1385/1-59259-358-5:161.

Construction of transgenic animals overproducing neurotrophins and their receptors.

Methods Mol Biol. 2001;169:167-91. doi: 10.1385/1-59259-060-8:167.

Northern blot analysis.

Methods Mol Biol. 1996;58:113-28. doi: 10.1385/0-89603-402-X:113.

Analysis of gene expression by northern blot.

Mol Biotechnol. 1994 Dec;2(3):227-42. doi: 10.1007/BF02745879.

Quantification of mRNA levels using ribonuclease protection assay.

Methods Mol Biol. 2001;169:65-79. doi: 10.1385/1-59259-060-8:65.

Northern blotting of RNA denatured in glyoxal without buffer recirculation.

Biotechniques. 1997 Apr;22(4):668-71. doi: 10.2144/97224st01.

引用本文的文献

PTT-quant: a new method for direct identification and absolute quantification of premature transcription termination events, following the example of bacterial riboswitches.

Appl Microbiol Biotechnol. 2022 Feb;106(4):1557-1570. doi: 10.1007/s00253-022-11809-1. Epub 2022 Feb 5.

Errors in CGAP xProfiler and cDNA DGED: the importance of library parsing and gene selection algorithms.

BMC Bioinformatics. 2011 Apr 15;12:97. doi: 10.1186/1471-2105-12-97.

Sizing up the future of microRNA analysis.

Anal Bioanal Chem. 2010 Nov;398(6):2535-49. doi: 10.1007/s00216-010-4018-8. Epub 2010 Aug 1.

Quantification of mRNA levels using northern blotting.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

Quantification of mRNA levels using northern blotting.

作者信息

机构信息

出版信息

相似文献

引用本文的文献