Grichko V P, Glick B R
Department of Biology, University of Waterloo, ON, Canada.
Can J Microbiol. 2000 Dec;46(12):1159-65. doi: 10.1139/w00-113.
Analysis of the DNA sequence upstream of the previously isolated Enterobacter cloacae UW4 ACC deaminase gene (Shah et al. 1998) suggests that this segment contains several features that are thought to be involved in the transcriptional regulation of this gene. These features include half of a CRP (cAMP receptor protein) binding site, an FNR (fumarate-nitrate reduction) regulatory protein binding site, an LRP (leucine responsive regulatory protein) binding site, and an LRP-like protein coding region. ACC deaminase activity was measured following growth of either various Escherichia coli strains carrying a plasmid that contained the Enterobacter cloacae UW4 ACC deaminase gene, or of Enterobacter cloacae UW4. Variables that were compared include aerobic versus anaerobic conditions, the presence and absence of ACC in the growth medium, addition of leucine to the medium, and bacterial strains that did or did not contain either lrp or fnr genes. The data reported are consistent with the involvement of most, if not all, of the above mentioned potential regulatory regions in the expression of ACC deaminase.
对先前分离出的阴沟肠杆菌UW4的ACC脱氨酶基因上游DNA序列（Shah等人，1998年）的分析表明，该片段包含几个被认为参与该基因转录调控的特征。这些特征包括CRP（环腺苷酸受体蛋白）结合位点的一半、FNR（延胡索酸-硝酸盐还原）调节蛋白结合位点、LRP（亮氨酸应答调节蛋白）结合位点以及一个LRP样蛋白编码区。在携带含有阴沟肠杆菌UW4的ACC脱氨酶基因的质粒的各种大肠杆菌菌株生长后，或者在阴沟肠杆菌UW4生长后，测量ACC脱氨酶活性。比较的变量包括需氧与厌氧条件、生长培养基中ACC的存在与否、向培养基中添加亮氨酸以及含有或不含有lrp或fnr基因的细菌菌株。所报道的数据与上述大多数（如果不是全部）潜在调控区域参与ACC脱氨酶表达的情况一致。
