Billotte W G, Hofmann M C
Department of Biology, The University of Dayton, Dayton, OH 45469, USA.
Biomed Sci Instrum. 1999;35:327-32.
We report here the establishment of a protocol to study the influence of mechanical stress on the behavior of primary human osteogenic cells. We first developed a method for the specific isolation of human bone marrow osteoprogenitors and studied their potential of differentiation into osteoblasts in vitro. Then, using NIH-3T3 cells as a model for cells of mesenchymal origin, we employed a parallel plate flow chamber apparatus to apply shear stress to cells in culture. This technique will be useful to determine the influence of shear stress on the rate of proliferation and differentiation of human osteoprogenitors and osteoblasts. Ultimately, this line of research will extend our knowledge on osteogenesis and bone remodeling.
我们在此报告一种研究机械应力对原代人成骨细胞行为影响的实验方案的建立。我们首先开发了一种特异性分离人骨髓骨祖细胞的方法，并研究了它们在体外分化为成骨细胞的潜力。然后，以NIH-3T3细胞作为间充质来源细胞的模型，我们使用平行板流动腔装置对培养中的细胞施加剪切应力。该技术将有助于确定剪切应力对人骨祖细胞和成骨细胞增殖和分化速率的影响。最终，这一系列研究将扩展我们对骨生成和骨重塑的认识。
