Chentanez V, Chittmittrapap S, Cheepsoonthorn P, Agthong S
Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
J Med Assoc Thai. 2000 Oct;83(10):1196-201.
Several previous studies have introduced classifications of Acetylcholinesterase (AChE) histochemical staining patterns in rectal suction biopsy performed in patients with Hirschsprung's disease. However, we introduce a new classification that is less complicated but shows the same age dependence as seen in previous studies. 135 rectal suction biopsies were submitted to histochemical staining for AChE activity and 88 specimens showed increased AChE activity. Therefore, we retrospectively analysed these 88 cases and could establish three patterns. Pattern I, presence of thick nerve trunks or coarse nerve fibers only in the muscularis mucosae and submucosa. This pattern was mainly seen in children aged 6 months or below. Pattern II, presence of abundant nerve fibers in all three layers of mucosa. This pattern was predominantly seen in children over 6 months of age. Pattern III, not predominant in any age group, showed positive nerve fibers in all three layers but, in one or more layers, the nerve fibers were sparse. Upon comparison with previous studies, we could observe the same age-pattern relationship. Thus, we propose this method of classification as a new tool to classify AChE histochemical staining patterns.
此前已有多项研究介绍了先天性巨结肠症患者直肠吸引活检中乙酰胆碱酯酶（AChE）组织化学染色模式的分类。然而，我们引入了一种新的分类方法，该方法不太复杂，但显示出与先前研究中相同的年龄依赖性。135份直肠吸引活检标本进行了AChE活性的组织化学染色，88份标本显示AChE活性增加。因此，我们对这88例病例进行了回顾性分析，并确定了三种模式。模式I，仅在黏膜肌层和黏膜下层存在粗大的神经干或粗大的神经纤维。这种模式主要见于6个月及以下的儿童。模式II，在黏膜的所有三层中均存在丰富的神经纤维。这种模式主要见于6个月以上的儿童。模式III，在任何年龄组中都不占主导地位，在所有三层中均显示阳性神经纤维，但在一层或多层中神经纤维稀疏。与先前的研究相比，我们可以观察到相同的年龄-模式关系。因此，我们建议将这种分类方法作为一种新的工具来对AChE组织化学染色模式进行分类。
