Kreiter S, Wehler T, Landt O, Huber C, Derigs H G, Hess G
III, Medical Department of Medicine, Hematology, Oncology and Pulmonology, Johannes Gutenberg-University, Mainz, Germany.
Tissue Antigens. 2000 Nov;56(5):449-52. doi: 10.1034/j.1399-0039.2000.560509.x.
Donor-recipient disparitiy of the minor histocompatibility antigen HA-1 is relevant for the development of graft-versus-host disease after HLA-matched sibling allogeneic bone marrow transplantation in HLA-A0201-positive individuals. Two different alleles of HA-1 with a single amino acid polymorphism have been identified. Here we describe a time- and cost-efficient method for HA-1 typing of genomic DNA, using site-specific hybridization probes with the LightCycler. This method was compared with standard techniques as sequencing or allele-specific polymerase chain reaction (PCR) and proved to be specific, reliable and reproducible. We conclude that HA-1-subtyping using fluorescent-labeled oligonucleotides represents a attractive method for the screening of samples before allogeneic transplantation in HLA-A0201-positive individuals.
次要组织相容性抗原HA-1的供受者差异与HLA-A0201阳性个体中HLA匹配的同胞异基因骨髓移植后移植物抗宿主病的发生相关。已鉴定出具有单个氨基酸多态性的两种不同的HA-1等位基因。在此，我们描述了一种使用LightCycler的位点特异性杂交探针，对基因组DNA进行HA-1分型的省时且经济高效的方法。该方法与测序或等位基因特异性聚合酶链反应（PCR）等标准技术进行了比较，结果证明具有特异性、可靠性和可重复性。我们得出结论，使用荧光标记寡核苷酸进行HA-1亚型分型是一种有吸引力的方法，可用于在HLA-A0201阳性个体进行异基因移植前筛选样本。
