Szarapińska-Kwaszewska J, Mikucki J
Zakład Mikrobiologii Farmaceutycznej Katedry Mikrobiologii Akademii Medycznej w Łodzi.
Med Dosw Mikrobiol. 2000;52(3):237-45.
The functional interchangeability of siderophores was tested among 62 strais belonging to 12 species of genus Enterococcus. Most investigated strains were from E. faecalis and E. faecium species. The majority of examined enterococcal strains appeared highly resistant to EDDA (ethylene-di-amine-di-ortho-hydroxyphenylacetic acid), therefore the group of sensitive strains involved only 11 used as indicator strains. The determination of interchangeability of siderophores within enterococcal strains was performed using EDDA-agar media into which the indicator strains were included. Test colonies (donor strains) were applied to the surface of the media to determine whether the indicator organisms could obtain the required iron for growth by utilizing chelators from the test colony. Only two strains: E. solitarius DSM 5634 and E. pseudoavium DSM 5632 did not demonstrate the ability to utilize siderophores synthesized by all investigated strains. The other tested indicator strains appeared to be recipients of siderophores from 20-52 donor enterococcal strains. The ability to exchange siderophores in enterococci was found as the feature characterizing individual strains.
在属于肠球菌属12个种的62株菌株中测试了铁载体的功能互换性。大多数被研究的菌株来自粪肠球菌和屎肠球菌种。大多数检测的肠球菌菌株对乙二胺二邻羟基苯乙酸(EDDA)表现出高度抗性,因此敏感菌株组仅包括11株用作指示菌株。在含有指示菌株的EDDA琼脂培养基中进行肠球菌菌株中铁载体互换性的测定。将测试菌落(供体菌株)接种到培养基表面,以确定指示生物是否可以通过利用来自测试菌落的螯合剂获得生长所需的铁。只有两株菌株:孤独肠球菌DSM 5634和伪鸟肠球菌DSM 5632没有表现出利用所有被研究菌株合成的铁载体的能力。其他测试的指示菌株似乎是来自20-52株供体肠球菌菌株铁载体的受体。发现肠球菌中铁载体交换能力是表征单个菌株的特征。