大鼠肝移植模型中吲哚胺2,3-双加氧酶核苷酸序列的鉴定。

Identification of the indoleamine 2,3-dioxygenase nucleotide sequence in a rat liver transplant model.

作者信息

Pan T L, Lin C L, Chen C L, Lin Y C, Gojo S, Lee T H, Wang Y H, Lord R, Lai C Y, Tsu L W, Tseng H P, Wu M L, Iwashita Y, Kitano S, Chiang K C, Hashimoto T, Sugioka A, Goto S

机构信息

Department of Surgery, Chang Gung Memorial Hospital, Niao-Sung, Kaohsiung, Taiwan.

出版信息

Transpl Immunol. 2000 Nov;8(3):189-94. doi: 10.1016/s0966-3274(00)00024-1.

DOI:10.1016/s0966-3274(00)00024-1

PMID:11147699

Abstract

A tryptophan catabolizer, indoleamine 2,3-dioxygenase (IDO) is highly expressed in the placenta and plays an essential role in maternal tolerance. Recent data have shown that the administration of an IDO inhibitor blocked not only maternal tolerance but also liver allograft tolerance. However, little is known about the induction of IDO in liver allografts, although a gene specific for tryptophan 2,3-dioxygenase (TDO) is believed to be expressed in the liver. In the present study, we investigated whether IDO is induced in liver allografts. Synthetic oligonucleotide primers based on the mouse IDO cDNA sequence were used to amplify RNA derived from livers of donor, syngeneic or allogeneic OLT rats. RNA encoding IDO was induced in the rat allogeneic liver after orthotopic liver transplantation (OLT), but not in syngeneic OLT. The rat nucleotide sequence of the RT-PCR products obtained from OLT livers revealed identities of 89% homology to the mouse IDO and of 68% to the human IDO. This study demonstrated the presence of RNA encoding IDO in allogeneic OLT livers, which may be involved in the immune response after liver transplantation.

摘要

色氨酸分解代谢酶吲哚胺2,3-双加氧酶（IDO）在胎盘中高度表达，并在母体免疫耐受中发挥重要作用。最近的数据表明，给予IDO抑制剂不仅会阻断母体免疫耐受，还会阻断肝移植耐受。然而，尽管人们认为肝脏中存在一种特异于色氨酸2,3-双加氧酶（TDO）的基因，但对于肝移植中IDO的诱导情况却知之甚少。在本研究中，我们调查了肝移植中是否会诱导IDO的产生。基于小鼠IDO cDNA序列合成的寡核苷酸引物，用于扩增来自供体、同基因或异基因原位肝移植（OLT）大鼠肝脏的RNA。原位肝移植后，大鼠异基因肝脏中诱导产生了编码IDO的RNA，但同基因原位肝移植中未出现这种情况。从原位肝移植肝脏获得的RT-PCR产物的大鼠核苷酸序列显示，与小鼠IDO的同源性为89%，与人类IDO的同源性为68%。本研究证明了异基因原位肝移植肝脏中存在编码IDO的RNA，这可能参与肝移植后的免疫反应。