Sladek J R, McConnel J, McNeill T H
Adv Exp Med Biol. 1978;113:241-50. doi: 10.1007/978-1-4684-8893-7_15.
A new method for the simultaneous visualization of brain peptides and monoamine neurotransmitters was employed to analyze the integrated morphology of hypothalamic catecholamines and neurophysins in young and old monkeys. Immunocytochemical analysis using bovine neurophysin revealed a dual papulation of light and dark stained cells in the paraventricular nucleus in young and old macaques. In general, both populations of neurons stained with less density in old macaques indicating the possibility of a reduced content of neurophysin. Further analysis using specific neurophysin antisera for vasopressin or oxytocin revealed an appreciable decrease in the number of vassopressin-containing perikarya in the 20 year monkey whereas oxytocin-synthesizing neurons did not show a similar change in numbers with age. Qualitatively, terminal innervation patterns of hypothalamic catecholamines remained strikingly constant in spite of marked reductions in dark-stained neurophysin perikarya of the paraventricular nucleus.
采用一种同时可视化脑肽和单胺神经递质的新方法，来分析幼年和老年猴子下丘脑儿茶酚胺和神经垂体素的整体形态。使用牛神经垂体素进行免疫细胞化学分析，发现在幼年和老年猕猴的室旁核中存在浅色和深色染色细胞的双重群体。总体而言，老年猕猴中这两种神经元群体的染色密度较低，表明神经垂体素含量可能降低。使用针对加压素或催产素的特异性神经垂体素抗血清进行的进一步分析显示，20岁猴子中含加压素的核周体数量明显减少，而合成催产素的神经元数量并未随年龄出现类似变化。定性地说，尽管室旁核中深色染色的神经垂体素核周体显著减少，但下丘脑儿茶酚胺的终末支配模式仍保持惊人的恒定。
