Okazaki Y, Shizuri Y
Marine Biotechnology Institute Co. Ltd, Shimizu Laboratory, 1900 Sodesi-cho, Shimizu-shi, Shizuoka 424-0037, Japan.
Dev Growth Differ. 2001 Feb;43(1):33-41. doi: 10.1046/j.1440-169x.2001.00552.x.
Serotonin and dopamine are involved in the attachment and metamorphosis of cypris larvae of barnacles. Aromatic L-amino acid decarboxylase (AADC) gene, the product of which catalyzes the synthesis of serotonin and dopamine from L-5-hydroxytryptophan and L-3,4-dihydroxyphenylalanine, respectively, was characterized. A DNA clone containing part of an AADC sequence was obtained from the genomic DNA library of the barnacle, Balanus amphitrite. This clone had four putative exons consisting of 226 amino acids with an identity of 63.2% and a similarity of 92.1% with human AADC. Northern blot analysis showed that AADC mRNA was expressed at all stages of barnacles: naupliar larvae, cypris larvae and adult barnacles. Two inducers of larval attachment and metamorphosis; that is, serotonin and extract of adult barnacles, obviously increased the expression of AADC mRNA at an early cypris larval stage. These results suggest that intracellular biosynthesis of serotonin, or dopamine, or both is at least partly involved in the control of the attachment and metamorphosis of cypris larvae.
血清素和多巴胺参与藤壶无节幼虫的附着和变态过程。对芳香族L-氨基酸脱羧酶（AADC）基因进行了表征，该基因的产物分别催化从L-5-羟色氨酸和L-3,4-二羟基苯丙氨酸合成血清素和多巴胺。从藤壶白脊藤壶的基因组DNA文库中获得了一个包含部分AADC序列的DNA克隆。该克隆有四个推定的外显子，由226个氨基酸组成，与人类AADC的同一性为63.2%，相似性为92.1%。Northern印迹分析表明，AADC mRNA在藤壶的所有阶段均有表达：无节幼虫、腺介幼虫和成年藤壶。两种幼虫附着和变态的诱导剂，即血清素和成年藤壶提取物，在腺介幼虫早期明显增加了AADC mRNA的表达。这些结果表明，血清素或多巴胺或两者的细胞内生物合成至少部分参与了腺介幼虫附着和变态的控制。
