Michael E
Z Parasitenkd. 1975;45(4):347-61. doi: 10.1007/BF00329824.
The macrogamete of Eimeria acervulina, lay and developed within the host cell in a parasitophorous vacuole. The cytoplasmic membrane of the host cell bordering the vacuole was not smooth, but it had numerous folds extending into the vacuole. These "intravacuolar folds" varied in depth and number in different sections. In some, the majority of the folds were disconnected from the host cell. Once disconnected, they evidently disintegrated forming the amorphous, particulate material present in the parasitophorous vacuole. The pellicle of the young macrogamete consisted of a single unit membrane with an osmiophilic material representing the second membrane. Two unit membranes were apparent at a later stage of development when the wall-forming bodies had been formed and amylopectin granules deposited. Two kinds of organelles were present on the surface of the macrogamete, typical micropores and invaginations of the pellicle. The micropores arose from an invagination of the outer membrane, which continued through the invagination without interruption. Irrespective of whether an inner membrane was present in the pellicle or not, a thickened cylindrical wall around the inner portion of the invagination was always present. Micropores appeared in large numbers in both micro- and macrogametocytes. As many as three micropores were seen in a surface area of 2 mu2. Invaginations arose in a similar manner by infolding of the pellicle. They differed from micropores in that the thickened cylindrical wall present around the inner portion of the micropore was absent, and also in that invaginations had no uniform appearance. They were of varying shapes, and lengths, varying from very short V-shaped to long and narrow. Micropores and invaginations take in nutrients in the form of particulate matter present in the parasitophorous vacuole, this material having been derived from the host-cell membranous "intravacuolar folds". The micropores function as cytostomes and the invaginations take in material by means of pinocytosis. Large numbers of intravacuolar tubules were seen at the surface of the macrogamete. They were present only at certain areas of the macrogamete and in groups and were connecting the parasite with the host cell. They were about 80-110 nm in diameter, and were seen to attain a length of up to 6 mu. Evidence was obtained indicating that the tubules transport free ribosomes from the host cell to the parasite. The ribosomes were seen to accumulate in "pockets" within the cytoplasm of the host cell, at the area where the tubules were connected.
堆型艾美耳球虫的大配子在寄生泡内的宿主细胞中产卵并发育。与泡相邻的宿主细胞的细胞质膜不光滑，有许多褶皱延伸到泡内。这些“泡内褶皱”在不同切片中的深度和数量各不相同。在一些切片中，大多数褶皱与宿主细胞分离。一旦分离，它们显然会解体，形成寄生泡中存在的无定形颗粒物质。幼大配子的表膜由一层单位膜和一层代表第二膜的嗜锇物质组成。在发育后期，当形成壁状体并沉积支链淀粉颗粒时，可以看到两层单位膜。大配子表面有两种细胞器，典型的微孔和表膜内陷。微孔由外膜内陷形成，内陷不间断地穿过外膜。无论表膜中是否存在内膜，内陷内部周围总是有一层增厚的圆柱形壁。微孔在小配子体和大配子体中大量出现。在2μm²的表面积内可以看到多达三个微孔。内陷以类似的方式由表膜内褶形成。它们与微孔的不同之处在于，微孔内部周围不存在增厚的圆柱形壁，而且内陷没有统一的外观。它们形状各异，长度不一，从非常短的V形到又长又窄。微孔和内陷以寄生泡中存在的颗粒物质的形式摄取营养，这种物质来源于宿主细胞膜的“泡内褶皱”。微孔起到胞口的作用，内陷通过胞饮作用摄取物质。在大配子表面可以看到大量泡内小管。它们只存在于大配子的某些区域，成组出现，将寄生虫与宿主细胞连接起来。它们的直径约为80 - 110nm，长度可达6μm。有证据表明，这些小管将游离核糖体从宿主细胞转运到寄生虫。可以看到核糖体在宿主细胞细胞质中与小管相连的区域的“口袋”中积累。
