Itoh M T, Ishizuka B, Kuribayashi Y, Abe Y, Sumi Y
Department of Chemistry, St. Marianna University School of Medicine, Kawasaki, Japan.
Exp Clin Endocrinol Diabetes. 2000;108(8):506-9. doi: 10.1055/s-2000-11004.
The concentrations of noradrenaline in preovulatory follicular fluid obtained from women undergoing in vitro fertilization were determined by high-performance liquid chromatography with fluorometric detection and compared with those in peripheral plasma obtained concurrently. All of the follicular fluid samples contained noradrenaline at concentrations substantially higher than those in the corresponding plasma samples. A positive correlation was found between noradrenaline levels in follicular fluid and plasma in each woman (n=11; r=0.952; p<0.001). However, there was no significant difference between noradrenaline concentrations in follicular fluid aspirated from follicles with or without an oocyte [mean+/-SEM, 0.207+/-0.002 ng/microl for follicular fluid samples with an oocyte (n=44), and 0.221+/-0.003 ng/microl for follicular fluid samples without an oocyte (n=13)]. The data indicate that noradrenaline accumulates in follicular fluid, supporting the physiological significance of noradrenaline in the local regulation of human ovarian functions.
采用高效液相色谱荧光检测法测定了接受体外受精的女性排卵前卵泡液中去甲肾上腺素的浓度，并与同期采集的外周血浆中的浓度进行比较。所有卵泡液样本中去甲肾上腺素的浓度均显著高于相应的血浆样本。在每位女性（n = 11；r = 0.952；p < 0.001）的卵泡液和血浆中去甲肾上腺素水平之间发现了正相关。然而，从有卵母细胞或无卵母细胞的卵泡中吸出的卵泡液中去甲肾上腺素浓度之间无显著差异[有卵母细胞的卵泡液样本（n = 44）的平均值±标准误为0.207±0.002 ng/μl，无卵母细胞的卵泡液样本（n = 13）为0.221±0.003 ng/μl]。数据表明去甲肾上腺素在卵泡液中蓄积，支持了去甲肾上腺素在人类卵巢功能局部调节中的生理意义。
