Servicio de Oftalmología, Hospital Miguel Servet, Zaragoza, España.
Arch Soc Esp Oftalmol. 2000 Jul;75(7):443-8.
To study the effect of topical ketorolac-tromethamine in the arachidonic acid metabolism on a model of endotoxin induced uveitis (EIU) in albino rabbits.
EIU was produced by intravitreal injection in the right eye with 10 ng LPS A Salmonella typhimurium endotoxin in 5ul saline solution. We used 60 animals (5 groups of 12 animals each). Control group (G-I) was injected with saline (5 ul); endotoxin group (G-II) was injected with 10 ng of ET; group III, IV and V were injected with the same amount of ET and treated with topical ketorolac-tromethamine every 6, 4 and 2 hours respectively. The animals were sacrificed 24 hours after the ET administration and we determined E2 prostaglandin and B4 leukotriene concentration in the aqueous humor.
In all treated groups with ketorolac-tromethamine we observed a significant reduction (p<0.05) in aqueous humor concentration of E2 prostaglandin without increase of the B4 leukotriene concentration comparing with endotoxin group.
Ketorolac tromethamine produced an inhibition of ciclooxigenase metabolism of arachidonic acid without increase of lypoxygenase pathway.
研究局部应用酮咯酸氨丁三醇对白化兔内毒素诱导性葡萄膜炎（EIU）模型中花生四烯酸代谢的影响。
通过右眼玻璃体内注射10 ng鼠伤寒沙门氏菌内毒素LPS溶于5 μl盐溶液来制备EIU。我们使用了60只动物（每组12只，共5组）。对照组（G-I）注射盐水（5 μl）；内毒素组（G-II）注射10 ng内毒素；III组、IV组和V组注射相同量的内毒素，并分别每6小时、4小时和2小时局部应用酮咯酸氨丁三醇进行治疗。在内毒素给药后24小时处死动物，我们测定房水中E2前列腺素和B4白三烯的浓度。
与内毒素组相比，在所有用酮咯酸氨丁三醇治疗的组中，我们观察到房水中E2前列腺素浓度显著降低（p<0.05），而B4白三烯浓度没有增加。
酮咯酸氨丁三醇对花生四烯酸的环氧化酶代谢产生抑制作用，而不增加脂氧合酶途径。
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