Gerber A, Struy H, Weiss G, Lippert H, Ansorge S, Schulz H U
Institute of Immunology, Otto von Guericke University Magdeburg, 39120 Magdeburg, Germany.
J Interferon Cytokine Res. 2000 Dec;20(12):1083-90. doi: 10.1089/107999000750053753.
Granulocyte colony-stimulating factor (G-CSF) preferentially stimulates growth and differentiation of neutrophil precursors and activates neutrophil functions. The aim of the present study was to investigate the functional response of the neutrophil to exogenous recombinant human G-CSF (rHuG-CSF) in nonneutropenic patients. In 30 surgical intensive care unit patients with severely impaired wound healing, leukocyte differential count, plasma G-CSF level, and a broad spectrum of neutrophil functions were monitored before (day 0), throughout (days 1 and 5), and at days 1 and 5 after stopping G-CSF treatment. G-CSF application resulted in a 3.5-fold increase in peripheral blood granulocyte count at day 5 of treatment. The mean plasma G-CSF level rose from 48 to a maximum of 2314 pg/ml at day 1 of G-CSF therapy. Neutrophil chemotaxis and stimulated lysozyme release were decreased throughout G-CSF treatment, whereas respiratory burst activity, phagocytic activity, and intracellular calcium concentration were enhanced by G-CSF. Neutrophil membrane depolarization remained unaffected. The increased count and activation state of neutrophils were associated with clinical improvement in most of these patients. Thus, G-CSF may be a useful adjuvant treatment for nonneutropenic patients with severely impaired wound healing.
粒细胞集落刺激因子（G-CSF）优先刺激中性粒细胞前体的生长和分化，并激活中性粒细胞的功能。本研究的目的是调查非中性粒细胞减少患者中性粒细胞对外源性重组人G-CSF（rHuG-CSF）的功能反应。在30例伤口愈合严重受损的外科重症监护病房患者中，在开始G-CSF治疗前（第0天）、治疗期间（第1天和第5天）以及停止G-CSF治疗后的第1天和第5天，监测白细胞分类计数、血浆G-CSF水平以及一系列中性粒细胞功能。在治疗第5天时，应用G-CSF使外周血粒细胞计数增加了3.5倍。在G-CSF治疗第1天时，血浆G-CSF平均水平从48 pg/ml升至最高2314 pg/ml。在整个G-CSF治疗期间，中性粒细胞趋化性和刺激后的溶菌酶释放减少，而呼吸爆发活性、吞噬活性和细胞内钙浓度则因G-CSF而增强。中性粒细胞膜去极化未受影响。这些患者中大多数中性粒细胞数量增加和激活状态与临床改善相关。因此，G-CSF可能是伤口愈合严重受损的非中性粒细胞减少患者的一种有用辅助治疗方法。
