Sansing G A, Davis N D, Diener U L
Appl Microbiol. 1975 Feb;29(2):300-2. doi: 10.1128/am.29.2.300-302.1975.
A rapid method for preparing cell-free extracts of Aspergillus ochraceus was developed. Mycelial mats were prefrozen in liquid nitrogen, ground to a fine powder in a cold mortar, and homogenized in an all-glass mechanical homogenizer. This method provided preparations averaging 43.0 mg of protein per g of mycelium (wet weight). The method was fast, efficient, and did not subject the extract to temperatures above 1 C or to heavy metals. The preparation method was suitable for studying a variety of in vitro fungal enzyme systems. Amylase, acid phosphate dehydrogenase, beta-glucosidase, beta fructofuranosidase, and trehalase activities were measurable in the preparations.
开发了一种制备赭曲霉无细胞提取物的快速方法。将菌丝垫在液氮中预冷冻，在冷研钵中研磨成细粉，然后在全玻璃机械匀浆器中匀浆。该方法制备的提取物平均每克菌丝体（湿重）含43.0毫克蛋白质。该方法快速、高效，且提取物不会暴露在高于1℃的温度或重金属环境中。该制备方法适用于研究多种体外真菌酶系统。在制备物中可检测到淀粉酶、酸性磷酸脱氢酶、β-葡萄糖苷酶、β-呋喃果糖苷酶和海藻糖酶的活性。
