Permiakov S E, Permiakov E A
Institute of Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Biofizika. 2000 Nov-Dec;45(6):990-1006.
Major results of the use of protein engineering methods in studies of calcium-binding proteins with the highest affinity for calcium and known three-dimensional structure (parvalbumin, calmodulin, troponin C, calbindin, recoverin, alpha-lactalbumin, and others) are presented. Specific features of recombinant calcium-binding proteins are discussed. Experiments with genetic introduction of fluorescent probes, tryptophan and tyrosine, into proteins are overviewed. Effects of mutations in different parts of protein molecules (calcium-binding loops, hydrophobic core, and others) on their structure and properties and attempts of creation of artificial calcium-binding sites are discussed.
本文介绍了在对钙具有最高亲和力且已知三维结构的钙结合蛋白（如小清蛋白、钙调蛋白、肌钙蛋白C、钙结合蛋白、恢复蛋白、α-乳白蛋白等）的研究中使用蛋白质工程方法的主要成果。讨论了重组钙结合蛋白的具体特征。概述了将荧光探针色氨酸和酪氨酸基因导入蛋白质的实验。讨论了蛋白质分子不同部位（钙结合环、疏水核心等）的突变对其结构和性质的影响以及创建人工钙结合位点的尝试。
