Zhou Z, Wang Y, Zhou Y, Zhang S
School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Chin Med J (Engl). 1998 Sep;111(9):847-50.
To evaluate the effect of gynostemma pentaphyllum mak (GP) on carcinomatous conversions of leukoplasia of the golden hamster cheek pouch induced by dimethylbenzanthracene (DMBA).
284 golden hamsters, ranging from 4 to 8 weeks of age, 80 to 100 grams in weight were randomly divided into three groups: control, model and experimental groups. Control group (8 hamsters) were sacrificed at week 0 and week 12 for control. Model group (123 hamsters) were pasted in bilateral cheek pouches with 0.5% DMBA, 3 times a week for establishment of experimental leukoplasia, and sacrificed at week 2 to week 9 (12-22 hamsters were sacrificed a week). Experimental group (153 hamsters) were divided into three subgroups. Subgroup A underwent simultaneous DMBA pasting and oral taking of GP, and were sacrificed at week 6 and week 9. Subgroup B underwent oral taking of GP for 12 weeks after completion of DMBA pasting. Subgroup C underwent DMBA pasting after completion of oral taking of GP for 12 weeks.
The earliest epithelial dysplasia and carcinoma in situ occurred at week 3 and week 6 after DMBA pasting. There were significant differences between subgroup B and model group for 3 weeks' administration of DMBA (P < 0.05), and between subgroup C and model group (P < 0.01), while there was no difference between subgroup B and model group for 6 week's administration of DMBA (P > 0.05).
GP could inhibit and reverse the carcinomatous conversions of leukoplasia of golden hamster cheek pouches, indicating its positive anticarcinogenic effect.
评估绞股蓝（GP）对二甲基苯并蒽（DMBA）诱导的金黄地鼠颊囊白斑癌变的影响。
284只4至8周龄、体重80至100克的金黄地鼠随机分为三组：对照组、模型组和实验组。对照组（8只地鼠）在第0周和第12周处死作为对照。模型组（123只地鼠）双侧颊囊涂抹0.5% DMBA，每周3次，以建立实验性白斑，在第2至9周处死（每周处死12 - 22只）。实验组（153只地鼠）分为三个亚组。A亚组在涂抹DMBA的同时口服GP，在第6周和第9周处死。B亚组在完成DMBA涂抹后口服GP 12周。C亚组在口服GP 12周后进行DMBA涂抹。
DMBA涂抹后最早的上皮发育异常和原位癌分别出现在第3周和第6周。DMBA给药3周时，B亚组与模型组之间有显著差异（P < 0.05），C亚组与模型组之间有显著差异（P < 0.01），而DMBA给药6周时，B亚组与模型组之间无差异（P > 0.05）。
GP可抑制并逆转金黄地鼠颊囊白斑的癌变，表明其具有积极的抗癌作用。
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