Zhang Xin-yan, Chen Xiao-fang, Sun Zheng, Miao Cong-cong, Ge Li-hua, Tian Zhen-chuan
Department of Periodontal and Mucosal Disease, Capital Medical University School of Stomatology, Beijng 100050, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Nov;46(11):678-83.
To evaluate the chemopreventive effects of boswellic acid and curcumin on 7,12-dimethyl benzanthracene(DMBA)-induced oral carcinogenesis in the hamster cheek pouch model.
Male Syrian golden hamsters (6 - 8 weeks old, 80 - 130 g in weight) were randomly divided into seven groups, with group A serving as the untreated negative control. The left cheek pouch of the remaining hamsters was topically treated with 0.5% DMBA in mineral oil three times a week for 6 weeks. They were then randomized to six groups with group B serving as a positive control and receiving no further treatment. Groups C-G were treated topically with 5, 10 mg/L boswellic acid, 5, 10 µmol/L curcumin, or the combination of 5 mg/L boswellic acid and 5 µmol/L curcumin three times per week for 18 weeks. The animals were injected with bromodeoxyuridine intraperitoneally at 50 mg/kg 2 h prior to killing. At the 25 th week all the hamsters were sacrificed and cheek pouch tissue was harvested. One half of the tissue was snap frozen in liquid nitrogen for analysis of arachidonic acid metabolites, and the other half was fixed in 10% phosphate-buffered saline(PBS)-buffered formalin for histopathological examination.
Six-weeks of DMBA followed by 18-weeks of topical application of boswellic acid and curcumin, both boswellic acid (5, 10 mg/L) and curcumin (5, 10 µmol/L) significantly inhibited the incidence from 93.8% to 73.9% (P > 0.05), numbers from 2.19 ± 0.98 to 1.13 ± 0.81 (P < 0.01) and size of visible tumors. Microscopically the incidence of squamous cell carcinoma and BrdU index were also significantly suppressed by boswellic acid and curcumin.
Both boswellic acid and curcumin were effective in preventing oral carcinogenesis in DMBA-induced hamster cheek pouch model.
在仓鼠颊囊模型中评估乳香酸和姜黄素对7,12 - 二甲基苯并蒽（DMBA）诱导的口腔癌发生的化学预防作用。
将雄性叙利亚金仓鼠（6 - 8周龄，体重80 - 130克）随机分为七组，A组作为未处理的阴性对照。其余仓鼠的左颊囊每周用含0.5% DMBA的矿物油局部处理三次，共6周。然后将它们随机分为六组，B组作为阳性对照，不再接受进一步处理。C - G组每周用5、10毫克/升乳香酸、5、10微摩尔/升姜黄素或5毫克/升乳香酸与5微摩尔/升姜黄素的组合局部处理三次，共18周。在处死前2小时，给动物腹腔注射50毫克/千克的溴脱氧尿苷。在第25周时，处死所有仓鼠并采集颊囊组织。一半组织在液氮中速冻用于分析花生四烯酸代谢物，另一半固定在10%磷酸盐缓冲盐水（PBS）缓冲甲醛中用于组织病理学检查。
DMBA处理6周后，再局部应用乳香酸和姜黄素18周，乳香酸（5、10毫克/升）和姜黄素（5、10微摩尔/升）均显著抑制了肿瘤发生率，从93.8%降至73.9%（P > 0.05），肿瘤数量从2.19 ± 0.98降至1.13 ± 0.81（P < 0.01），并减小了可见肿瘤的大小。显微镜下，乳香酸和姜黄素也显著抑制了鳞状细胞癌的发生率和BrdU指数。
在DMBA诱导的仓鼠颊囊模型中，乳香酸和姜黄素均能有效预防口腔癌发生。
