Hoshi K, Ejiri S, Ozawa H
First Department of Oral Anatomy, Niigata University Faculty of Dentistry, 2-5274, Gakkocho-dori, Niigata 951-8514, Japan.
Kaibogaku Zasshi. 2000 Oct;75(5):457-65.
Primary calcification in embryonic ossification occurs as follows: crystallization within matrix vesicles, formation of calcified nodules, and finally the establishment of expansive calcified matrix. However, the participation of the matrix vesicles in other types of bone calcification, such as bone formation during bone remodeling in adults has not been examined sufficiently. We introduce our recent observations on the presence of matrix vesicles in aged bones. In addition, although it is well known that the extracellular fluid supersaturates the calcification crystal, hydroxyapatite, the specific mechanisms by which bone matrix calcify remain unclear. In order to further approach the mechanisms of bone matrix calcification, we also review ultrastructural and localizational alterations of the matrix organics according to the progression of calcification, and an evaluation of mineral micro-environment in the calcifying sites by energy-filter transmission electron microscopy.
在基质小泡内结晶，形成钙化结节，最后形成扩展的钙化基质。然而，基质小泡在其他类型的骨钙化中的参与情况，如成人骨重塑过程中的骨形成，尚未得到充分研究。我们介绍了我们最近关于老年骨中基质小泡存在情况的观察结果。此外，尽管众所周知细胞外液使钙化晶体羟基磷灰石过饱和，但骨基质钙化的具体机制仍不清楚。为了进一步探究骨基质钙化的机制，我们还根据钙化进程回顾了基质有机物的超微结构和定位变化，以及通过能量过滤透射电子显微镜对钙化部位矿物质微环境的评估。
