溶液中连续共多肽的合成与构象研究：聚（L-脯氨酰-L-α-苯甘氨酰-L-脯氨酸）

Synthesis and conformational studies in solution of sequential copolypeptides: poly(L-prolyl-L-alpha-phenylglycyl-L-proline).

作者信息

Palumbo M, Rodin R L, Goodman M

出版信息

Biochemistry. 1975 Feb 11;14(3):485-91. doi: 10.1021/bi00674a004.

Abstract

High molecular weight poly(L-porlyl-L-alpha-phenylglycyl-L-proline) was synthesized by condensation of L-prolyl-L-alpha-phenylglycyl-L-proline pentachlorophenyl ester in N,N-dimethylformamide. The solution properties of this material have been investigated by circular dichroism and 220-MHz nuclear magnetic resonance spectroscopy in solvents such as trifluoroethanol (F3EtOH), chloroform, trifluoroacetic acid (F3CCOOH), chloroform-F3CCOOH and F3EtOH-F3CCOOH mixtures. Ordered structures can be formed in F3EtOH and chloroform, which we believe to be similar to the poly-L-proline II form. The ordered form easily undergoes a conformational transition to an unordered form upon addition of small amounts of F3CCOOH to the chloroform solution. The ordered structure is not disrupted upon addition of 12 per cent F3CCOOH to a F3EtOH solution of the polypeptide.

摘要

通过L-脯氨酰-L-α-苯基甘氨酰-L-脯氨酸五氯苯基酯在N，N-二甲基甲酰胺中的缩合反应合成了高分子量聚（L-脯氨酰-L-α-苯基甘氨酰-L-脯氨酸）。已通过圆二色性和220兆赫核磁共振光谱法在诸如三氟乙醇（F3EtOH）、氯仿、三氟乙酸（F3CCOOH）、氯仿-F3CCOOH和F3EtOH-F3CCOOH混合物等溶剂中研究了该材料的溶液性质。在F3EtOH和氯仿中可形成有序结构，我们认为其类似于聚-L-脯氨酸II型。在向氯仿溶液中加入少量F3CCOOH后，有序形式容易发生构象转变为无序形式。向该多肽的F3EtOH溶液中加入12%的F3CCOOH时，有序结构不会被破坏。