Casaz Paul, Happel Anne, Keithan Joann, Read Dorothy L, Strain Steven R, Levy Stuart B
Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1.
Microbiology (Reading). 2001 Feb;147(Pt 2):355-361. doi: 10.1099/00221287-147-2-355.
The locations of two mutations that prevent adhesion of Pseudomonas fluorescens Pf0-1 to sand columns and seeds (adn, adhesion) were identified. Both lie in a single gene showing homology to the NtrC/NifA family of transcription activators. The predicted 55 kDa protein encoded by adnA is most closely related to activators involved in expression of flagellar proteins, consistent with the lack of flagella in adnA strains. Constitutive adnA expression restored motility and adhesion to an adnA strain, demonstrating that the observed phenotypes are due to lack of AdnA and not a consequence of other mutations or polar effects of mutations in adnA on other genes.
鉴定出了两种阻止荧光假单胞菌Pf0-1附着于沙柱和种子的突变（adn，附着）的位置。这两种突变都位于一个与转录激活因子NtrC/NifA家族具有同源性的单一基因中。adnA编码的预测55 kDa蛋白与参与鞭毛蛋白表达的激活因子关系最为密切，这与adnA菌株中缺乏鞭毛一致。组成型adnA表达恢复了adnA菌株的运动性和附着能力，表明观察到的表型是由于缺乏AdnA，而不是其他突变或adnA突变对其他基因的极性效应的结果。
