Prigozy T I, Naidenko O, Qasba P, Elewaut D, Brossay L, Khurana A, Natori T, Koezuka Y, Kulkarni A, Kronenberg M
Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA.
Science. 2001 Jan 26;291(5504):664-7. doi: 10.1126/science.291.5504.664.
The requirement for processing glycolipid antigens in T cell recognition was examined with mouse CD1d-mediated responses to glycosphingolipids (GSLs). Although some disaccharide GSL antigens can be recognized without processing, the responses to three other antigens, including the disaccharide GSL Gal(alpha1-->2)GalCer (Gal, galactose; GalCer, galactosylceramide), required removal of the terminal sugars to permit interaction with the T cell receptor. A lysosomal enzyme, alpha-galactosidase A, was responsible for the processing of Gal(alpha1-->2)GalCer to generate the antigenic monosaccharide epitope. These data demonstrate a carbohydrate antigen processing system analogous to that used for peptides and an ability of T cells to recognize processed fragments of complex glycolipids.
利用小鼠CD1d介导的对糖鞘脂（GSLs）的反应，研究了T细胞识别中处理糖脂抗原的要求。尽管一些二糖GSL抗原无需处理就能被识别，但对其他三种抗原的反应，包括二糖GSL Gal(α1→2)GalCer（Gal，半乳糖；GalCer，半乳糖基神经酰胺），需要去除末端糖以允许与T细胞受体相互作用。一种溶酶体酶，α-半乳糖苷酶A，负责处理Gal(α1→2)GalCer以产生抗原性单糖表位。这些数据证明了一种类似于用于肽的碳水化合物抗原加工系统，以及T细胞识别复杂糖脂加工片段的能力。
