裂殖酵母中，S期细胞周期蛋白Cig2在G1-S边界的表达高峰需要后期促进复合物（APC）和Skp1-Cul1-F-box蛋白复合物（SCF）泛素连接酶。

The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases.

作者信息

Yamano H, Kitamura K, Kominami K, Lehmann A, Katayama S, Hunt T, Toda T

机构信息

Laboratory of Cell Cycle Control, Imperial Cancer Research Fund, South Mimms, Herts EN6 3LD, United Kingdom.

出版信息

Mol Cell. 2000 Dec;6(6):1377-87. doi: 10.1016/s1097-2765(00)00135-0.

DOI:10.1016/s1097-2765(00)00135-0

PMID:11163211

Abstract

We describe a novel set of oscillation mechanisms for the fission yeast S phase cyclin Cig2, which contains an authentic destruction box and is destroyed at anaphase via the APC/cyclosome (APC/C). Unlike the mitotic cyclin Cdc13, however, Cig2 mRNA and protein peak at the G1/S boundary and decline to low levels in G2 and M phases. We show here that SCF(Pop1, Pop2) plays a role in transcriptional periodicity, as pop mutations result in constitutive cig2(+) transcripts. The instability of Cig2 during G2 and M is independent of either the APC/C or Pop1/Pop2, but requires Skp1, a core component of SCF. These data indicate that the APC/C and SCF control Cig2 levels differentially at different stages of the cell cycle.

摘要

我们描述了裂殖酵母S期细胞周期蛋白Cig2的一组新的振荡机制，Cig2含有一个真正的破坏框，并在后期通过后期促进复合物/细胞周期体（APC/C）被降解。然而，与有丝分裂细胞周期蛋白Cdc13不同，Cig2 mRNA和蛋白在G1/S边界达到峰值，并在G2和M期降至低水平。我们在此表明，SCF（Pop1，Pop2）在转录周期性中起作用，因为pop突变导致组成型cig2（+）转录本。Cig2在G2和M期的不稳定性与APC/C或Pop1/Pop2无关，但需要Skp1，它是SCF的核心成分。这些数据表明，APC/C和SCF在细胞周期的不同阶段差异地控制Cig2的水平。