Gaasenbeek C P, Moll L, Cornelissen J B, Vellema P, Borgsteede F H
Institute for Animal Science and Health (ID-Lelystad), PO Box 65, 8200 AB, Lelystad, Netherlands.
Vet Parasitol. 2001 Feb;95(1):37-43. doi: 10.1016/s0304-4017(00)00413-1.
The efficacy of triclabendazole in sheep experimentally infected with Fasciola hepatica was studied. Two groups of 12 lambs were infected with a susceptible (S) or a resistant (R) strain of F. hepatica. Eight weeks after infection, six lambs of each group (ST and RT) were treated with triclabendazole (10mg/kg). The other lambs were used as untreated controls (SC and RC). The parameters studied were: GLDH, gamma-GT, ELISA measuring antibodies against recombinant cathepsin-L(1) and eggs per gram faeces (epg). The lambs were slaughtered 16 weeks after infection and the number of flukes counted. The GLDH, gamma-GT levels and the OD value of the ELISA decreased as a result of the treatment in group ST. Patent infections were observed in all animals of groups SC, RT and RC. In group ST, occasionally a few eggs were found in five lambs. The percentage of flukes was 31.3 in SC and 37.6 in RC. In the treated groups ST and RT, the percentage of flukes was 0.06 and 33.6, respectively. These results corresponded to efficacies of 99.8% in the susceptible and 10.8% in the resistant strain. Since the resistant strain was isolated from a mixed cattle and sheep farm, it confirms the presence of triclabendazole resistance in the Netherlands.
研究了三氯苯达唑对实验感染肝片吸虫的绵羊的疗效。两组各12只羔羊分别感染肝片吸虫的敏感（S）株或抗性（R）株。感染8周后，每组6只羔羊（ST和RT）用三氯苯达唑（10mg/kg）治疗。其他羔羊用作未治疗的对照（SC和RC）。研究的参数包括：谷氨酸脱氢酶（GLDH）、γ-谷氨酰转移酶（γ-GT）、检测抗重组组织蛋白酶-L(1)抗体的酶联免疫吸附测定（ELISA）以及每克粪便中的虫卵数（epg）。感染16周后宰杀羔羊并计数吸虫数量。ST组治疗后GLDH、γ-GT水平及ELISA的光密度值下降。SC、RT和RC组的所有动物均出现显性感染。在ST组，偶尔在5只羔羊中发现少量虫卵。SC组和RC组的吸虫百分比分别为31.3%和37.6%。在治疗组ST和RT中，吸虫百分比分别为0.06%和33.6%。这些结果对应的敏感株疗效为99.8%，抗性株疗效为10.8%。由于抗性株是从一个牛羊混合养殖场分离出来的，这证实了荷兰存在三氯苯达唑抗性。
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