Antoniades H N
Endocrinology. 1975 Mar;96(3):799-802. doi: 10.1210/endo-96-3-799.
[125I]-growth hormone, injected in rats, was converted into high molecular weight forms (70,000 to over 200,000 daltons) as judged by gel filtration of blood serum samples collected after the injection. These forms were reactive with antisera against growth hormone, although their immunoreactivity was only 50% of that of control, single component [125I]growth hormone. Their molecular weight was not affected significantly by denaturation with sodium dodecyl sulfate. Incubation of [125I]growth hormone with blood serum in vitro did not result in the production of high molecular weight forms.
给大鼠注射[125I]生长激素后，通过对注射后采集的血清样本进行凝胶过滤判断，其转化为了高分子量形式（70,000至超过200,000道尔顿）。这些形式与抗生长激素抗血清有反应，尽管它们的免疫反应性仅为对照单一组分[125I]生长激素的50%。它们的分子量不受十二烷基硫酸钠变性的显著影响。在体外将[125I]生长激素与血清一起孵育不会产生高分子量形式。
