Quiding-Järbrink M, Lundin B S, Lönroth H, Svennerholm A M
Departments of Medical Microbiology and Immunology and Surgery, Göteborg University, Göteborg, Sweden.
Clin Exp Immunol. 2001 Jan;123(1):81-7. doi: 10.1046/j.1365-2249.2001.01427.x.
In order to characterize T cell responses in human Helicobacter pylori infection, we have examined proliferative responses and cytokine production by CD4+ and CD8+ T cells isolated from duodenal ulcer patients and asymptomatic H. pylori carriers, after activation with some H. pylori antigens that may be important in disease development. For control purposes, T cells from uninfected volunteers were also examined. The different H. pylori antigens induced only modest proliferative responses in circulating CD4+ and CD8+ T cells from both H. pylori-infected and uninfected individuals. However, circulating T cells from H. pylori-infected subjects produced larger amounts of interferon-gamma (IFN-gamma) in response to the Helicobacter antigens than did T cells from uninfected volunteers. Furthermore, CD8+ T cells produced larger amounts of IFN-gamma than did CD4+ T cells, on a per cell basis. Most IFN-gamma-producing cells from both infected and uninfected volunteers appeared to be naive T cells expressing CD45RA. Increased production of IL-4 and IL-5 was, on the other hand, only seen in a few instances after stimulation of isolated CD4+ and CD8+ T cells. Stimulation of freshly isolated gastric T cells with the different H. pylori antigens did not result in increased proliferation or cytokine production. In conclusion, our results show that several different purified H. pylori antigens induce production of IFN-gamma, preferentially by CD8+ cells. Therefore, they suggest that IFN-gamma-secreting CD8+ cells contribute significantly to the cytokine response induced by H. pylori infection.
为了描述人类幽门螺杆菌感染中的T细胞反应，我们检测了从十二指肠溃疡患者和无症状幽门螺杆菌携带者中分离出的CD4+和CD8+ T细胞在被一些可能在疾病发展中起重要作用的幽门螺杆菌抗原激活后的增殖反应和细胞因子产生情况。为作对照，还检测了未感染志愿者的T细胞。不同的幽门螺杆菌抗原仅在幽门螺杆菌感染和未感染个体的循环CD4+和CD8+ T细胞中诱导出适度的增殖反应。然而，与未感染志愿者的T细胞相比，幽门螺杆菌感染受试者的循环T细胞对幽门螺杆菌抗原产生了更多的干扰素-γ（IFN-γ）。此外，按每个细胞计算，CD8+ T细胞产生的IFN-γ比CD4+ T细胞更多。来自感染和未感染志愿者的大多数产生IFN-γ的细胞似乎是表达CD45RA的初始T细胞。另一方面，在分离的CD4+和CD8+ T细胞受到刺激后，仅在少数情况下观察到IL-4和IL-5产生增加。用不同的幽门螺杆菌抗原刺激新鲜分离的胃T细胞并未导致增殖增加或细胞因子产生增加。总之，我们的结果表明，几种不同的纯化幽门螺杆菌抗原可诱导IFN-γ产生，且优先由CD8+细胞产生。因此，这些结果提示分泌IFN-γ的CD8+细胞对幽门螺杆菌感染诱导的细胞因子反应有显著贡献。
