Nacken W, Lekstrom-Himes J A, Sorg C, Manitz M P
Institute of Experimental Dermatology, University of Münster, Von-Esmarch-Str. 56, D-48149 Münster, Germany.
J Cell Biochem. 2001;80(4):606-16.
The genomic locus of the mouse S100A9 (MRP14) gene, a myeloid expressed gene belonging to the S100 family, is split in three exons and two introns. Insertions of B1 like and LINE elements as well as several sequence repeat structures are scattered over the gene suggesting that this region of the S100 gene cluster has been the subject of a high mutational activity in mouse evolution. The insertions may represent molecular footprints of a recently postulated inversion event, which resulted in a rearrangement of the S100 gene cluster in mouse compared to man. Deletion analysis of the promoter reveals, that a 1200 bp fragment is able to direct a cell type-specific expression of a reporter gene in granulocytic 32D cells. Unexpectedly, the myeloid-specific transcription factor C/EBPepsilon is not needed for the transcriptional upregulation of the S100A9 and S100A8 genes in neutrophils. The data described here provide further insights into the evolution of the S100 gene cluster and into the myeloid-specific regulation of the murine S100A9 gene expression.
小鼠S100A9（MRP14）基因的基因组位点，属于S100家族的一个髓系表达基因，被分为三个外显子和两个内含子。类似B1的元件和长散在核元件（LINE）以及几个序列重复结构插入在整个基因中，这表明S100基因簇的这个区域在小鼠进化过程中经历了高突变活性。这些插入可能代表了最近推测的倒位事件的分子印记，与人类相比，该事件导致了小鼠S100基因簇的重排。启动子的缺失分析表明，一个1200 bp的片段能够在粒细胞32D细胞中指导报告基因的细胞类型特异性表达。出乎意料的是，中性粒细胞中S100A9和S100A8基因的转录上调不需要髓系特异性转录因子C/EBPε。这里描述的数据为S100基因簇的进化以及小鼠S100A9基因表达的髓系特异性调控提供了进一步的见解。
