Rizki M, Amrani S, Creus A, Xamena N, Marcos R
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Environ Mol Mutagen. 2001;37(1):70-5.
The genotoxic activity of three selenium compounds (sodium selenite, sodium selenate, and selenious acid) and the antigenotoxic effects of sodium selenite in combination with the chromium compound potassium dichromate were studied using the wing spot test of Drosophila melanogaster. This assay is based on the principle that the loss of heterozygosity of suitable recessive markers, multiple wing hairs (mwh) and flare-3 (flr[3]), can lead to the formation of mutant clones of larval cells, which are then expressed as spots on the wings of the adult flies. Pretreatment and chronic cotreatment was comparatively used for the antigenotoxicity study. From the results obtained, it was evident that all selenium compounds are unable to increase the frequency of any of the three categories of spots recorded (small, large, and twin spots). Nevertheless, the antigenotoxic effects of sodium selenite were clearly demonstrated, in both cotreatment and pretreatment, by a complete suppression of those clones induced by potassium dichromate. Therefore, the D. melanogaster wing spot test was revealed to be a good assay, not only for evaluating genotoxic activity but also for detecting antigenotoxic effects in vivo.
利用黑腹果蝇的翅斑试验，研究了三种硒化合物（亚硒酸钠、硒酸钠和亚硒酸）的遗传毒性活性以及亚硒酸钠与铬化合物重铬酸钾联合使用时的抗遗传毒性作用。该试验基于这样的原理：合适的隐性标记，即多翅毛（mwh）和flare - 3（flr[3]）的杂合性丧失，可导致幼虫细胞突变克隆的形成，这些克隆随后在成年果蝇的翅膀上表现为斑点。预处理和慢性联合处理被用于比较抗遗传毒性研究。从获得的结果来看，很明显所有的硒化合物都不能增加所记录的三类斑点（小斑点、大斑点和双斑点）中的任何一类的频率。然而，在联合处理和预处理中，亚硒酸钠的抗遗传毒性作用都通过完全抑制重铬酸钾诱导的那些克隆而得以清楚地证明。因此，黑腹果蝇翅斑试验不仅被证明是评估遗传毒性活性的良好试验，而且也是检测体内抗遗传毒性作用的良好试验。
