一种合成APTRA指标的新方法。

A new approach to the synthesis of APTRA indicators.

作者信息

Otten P A, London R E, Levy L A

机构信息

National Institute of Environmental Health Sciences, Laboratory of Structural Biology, P.O. Box 12233, MR-01, Research Triangle Park, North Carolina 27709, USA.

出版信息

Bioconjug Chem. 2001 Jan-Feb;12(1):76-83. doi: 10.1021/bc000069w.

DOI:10.1021/bc000069w

PMID:11170369

Abstract

We have devised a general synthesis of Mg(2+) indicators which is based on the aminophenol triacetic acid (APTRA) structure. The key step is a palladium-catalyzed coupling reaction of a precursor of the APTRA ligand with a fluorescent group. This strategy resulted in new ratioable fluorescent APTRA indicators and the finding that the fluorescence response of these indicators is different for Mg(2+) and Ca(2+) in some cases. We believe that this represents a generally useful approach for combining fluorophore and chelator functionalities.

摘要

我们设计了一种基于氨基酚三乙酸（APTRA）结构的Mg(2+)指示剂通用合成方法。关键步骤是APTRA配体前体与荧光基团的钯催化偶联反应。该策略产生了新的可用于比率测定的荧光APTRA指示剂，并且发现这些指示剂在某些情况下对Mg(2+)和Ca(2+)的荧光响应不同。我们认为这代表了一种将荧光团和螯合剂功能相结合的普遍有用的方法。