Mathai E, Kaufmann M E, Richard V S, John G, Brahmadathan K N
Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, Tamil Nadu, 632004, India.
J Hosp Infect. 2001 Feb;47(2):159-62. doi: 10.1053/jhin.2000.0906.
In an attempt to define the epidemiology of Acinetobacter baumannii infection, 27 isolates, obtained from hospital-acquired respiratory infections, were typed using random amplified polymorphic DNA (RAPD) profile and antimicrobial susceptibility patterns. Ten different patterns were obtained with ERIC2 primer: 14 isolates had a similar profile representing a single strain. Within RAPD types, isolates could be further classified based on their antibiogram; however, strains of different types had similar antibiograms. This study showed that many different genetic types of A. baumannii are prevalent in our hospital. While antibiograms alone are not sufficiently discriminatory, RAPD typing helps in identifying outbreaks and in assessing infection control procedures within a hospital.
为了明确鲍曼不动杆菌感染的流行病学特征,对从医院获得性呼吸道感染中分离出的27株菌株进行了随机扩增多态性DNA(RAPD)分型和抗菌药物敏感性模式分析。使用ERIC2引物获得了10种不同的模式:14株菌株具有相似的图谱,代表单一菌株。在RAPD类型中,分离株可根据其抗菌谱进一步分类;然而,不同类型的菌株具有相似的抗菌谱。本研究表明,多种不同基因类型的鲍曼不动杆菌在我院普遍存在。虽然仅靠抗菌谱不足以进行区分,但RAPD分型有助于识别暴发情况并评估医院内的感染控制措施。