Ece Gulfem, Erac Bayri, Yurday Cetin Hasan, Ece Cem, Baysak Aysegul
Department of Medical Microbiology, School of Medicine, Izmir University, Izmir, Turkey.
Department of Pharmaceutical Microbiology, School of Pharmacy, Ege University, Izmir, Turkey.
Jundishapur J Microbiol. 2015 Feb 20;8(2):e15612. doi: 10.5812/jjm.15612. eCollection 2015 Feb.
Acinetobacter baumannii is an opportunistic pathogen, related with nosocomial infections such as bacteremia, urinary tract infections, and ventilator-associated pneumonia. Multidrug resistant (MDR) A. baumannii strains are first line causes of infection, especially in patients hospitalized at intensive care units (ICUs). Infection with MDR A. baumannii strains has a longer duration at ICUs and hospitals. There are studies using molecular methods which can differentiate MDR A. baumannii strains at the clonal level. This helps controlling these resistant strains and prevents their epidemy.
The aim of our study was to investigate the antimicrobial susceptibility and clonal relationship between the A. baumannii strains isolated from our ICU.
The identification and antimicrobial susceptibility of 33 A. baumannii strains were performed by automatized Vitek version 2.0. The clonal relationship among A. baumannii strains was analyzed using enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR).
A total of 33 A. baumannii strains were included in this study. A. baumannii complex strains were classified into seven clusters based on the fingerprint results. Our results revealed that two main clusters were responsible for the prevalence of A. baumannii complex strains at the ICU.
MDR A. baumannii strains cause an increment in morbidity and mortality, particularly in ICUs. The use of molecular epidemiological methods can help us with the detection of the pathogen and preventing from spreading of these resistant strains.
鲍曼不动杆菌是一种机会致病菌，与医院感染如菌血症、尿路感染和呼吸机相关性肺炎有关。多重耐药（MDR）鲍曼不动杆菌菌株是感染的首要原因，尤其是在重症监护病房（ICU）住院的患者中。MDR鲍曼不动杆菌菌株感染在ICU和医院中的持续时间更长。有研究使用分子方法可在克隆水平区分MDR鲍曼不动杆菌菌株。这有助于控制这些耐药菌株并防止其流行。
我们研究的目的是调查从我们ICU分离的鲍曼不动杆菌菌株的抗菌药敏性和克隆关系。
33株鲍曼不动杆菌菌株的鉴定和抗菌药敏性通过自动化Vitek 2.0版进行。使用肠杆菌基因间重复一致序列（ERIC）聚合酶链反应（PCR）分析鲍曼不动杆菌菌株之间的克隆关系。
本研究共纳入33株鲍曼不动杆菌菌株。根据指纹图谱结果，鲍曼不动杆菌复合菌株分为七个簇。我们的结果显示，两个主要簇导致了ICU中鲍曼不动杆菌复合菌株的流行。
MDR鲍曼不动杆菌菌株导致发病率和死亡率增加，尤其是在ICU中。使用分子流行病学方法可以帮助我们检测病原体并防止这些耐药菌株的传播。
