新加坡株斜带石斑鱼神经坏死病毒RNA1和RNA2全核苷酸序列的测定

Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain.

作者信息

Tan Corrina, Huang Bin, Chang Siow Foong, Ngoh Gim Hue, Munday Barry, Chen Shih Chu, Kwang Jimmy

机构信息

Institute of Molecular Agrobiology, The National University of Singapore, 1 Research Link, Singapore1176041.

Agri-food and Veterinary Authority, Animal Health Service Branch, Central Veterinary Laboratory, 60 Sengkang East Way, Singapore5485962.

出版信息

J Gen Virol. 2001 Mar;82(Pt 3):647-653. doi: 10.1099/0022-1317-82-3-647.

DOI:10.1099/0022-1317-82-3-647

PMID:11172107

Abstract

The complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus (GGNNV), Singapore strain, were determined. 5' RACE and RNA ligation were used to obtain the complete nucleotide sequences of the 5' and 3' non-coding regions (NCRs). GGNNV RNA1 was determined to be 3103 nt long, containing an ORF of 982 aa, while GGNNV RNA2 was determined to be 1433 nt long, containing an ORF of 338 aa. Both GGNNV RNAs are longer than those of other published betanodavirus sequences and the additional nucleotides were located within the NCRs. Analysis of GGNNV RNA2 revealed that it is closely related to red-spotted grouper nervous necrosis virus and that both grouper viruses share the same neutralization epitope. Predicted domains for six RNA-dependent RNA polymerase motifs and two putative ORFs (proteins B1 and B2) were confirmed by sequence analysis of GGNNV RNA1.

摘要

测定了来自新加坡株驼背鲈神经坏死病毒（GGNNV）的RNA1和RNA2的完整核苷酸序列。采用5′RACE和RNA连接法获得了5′和3′非编码区（NCR）的完整核苷酸序列。GGNNV RNA1长度为3103 nt，包含一个982个氨基酸的开放阅读框（ORF），而GGNNV RNA2长度为1433 nt，包含一个338个氨基酸的ORF。两种GGNNV RNA均比其他已发表的β-诺达病毒序列长，额外的核苷酸位于NCR内。对GGNNV RNA2的分析表明，它与红斑石斑鱼神经坏死病毒密切相关，且两种石斑鱼病毒具有相同的中和表位。通过对GGNNV RNA1的序列分析，证实了六个依赖RNA的RNA聚合酶基序的预测结构域以及两个推定的开放阅读框（蛋白B1和B2）。